1k2a

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(New page: 200px<br /> <applet load="1k2a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k2a, resolution 1.00&Aring;" /> '''Modified Form of Eo...)
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[[Image:1k2a.gif|left|200px]]<br />
 
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<applet load="1k2a" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k2a, resolution 1.00&Aring;" />
 
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'''Modified Form of Eosinophil-derived Neurotoxin'''<br />
 
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==Overview==
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==Modified Form of Eosinophil-derived Neurotoxin==
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The crystal structure of a post-translationally modified form of, eosinophil-derived neurotoxin (EDN) with four extra residues on its N, terminus ((-4)EDN) has been solved and refined at atomic resolution (1 A)., Two of the extra residues can be placed unambiguously, while the density, corresponding to two others is poor. The modified N terminus appears to, influence the position of the catalytically important His129, possibly, explaining the diminished catalytic activity of this variant. However, (-4)EDN has been shown to be cytotoxic to a Kaposi's sarcoma tumor cell, line and other endothelial cell lines. Analysis of the structure and, function suggests that the reason for cytotoxicity is most likely due to, cellular recognition by the N-terminal extension, since the intrinsic, activity of the enzyme is not sufficient for cytotoxicity and the, N-terminal extension does not affect the conformation of EDN.
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<StructureSection load='1k2a' size='340' side='right'caption='[[1k2a]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k2a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K2A FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k2a OCA], [https://pdbe.org/1k2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k2a RCSB], [https://www.ebi.ac.uk/pdbsum/1k2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k2a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS2_HUMAN RNAS2_HUMAN] This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities.<ref>PMID:3458170</ref> <ref>PMID:12578357</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k2a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k2a ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of a post-translationally modified form of eosinophil-derived neurotoxin (EDN) with four extra residues on its N terminus ((-4)EDN) has been solved and refined at atomic resolution (1 A). Two of the extra residues can be placed unambiguously, while the density corresponding to two others is poor. The modified N terminus appears to influence the position of the catalytically important His129, possibly explaining the diminished catalytic activity of this variant. However, (-4)EDN has been shown to be cytotoxic to a Kaposi's sarcoma tumor cell line and other endothelial cell lines. Analysis of the structure and function suggests that the reason for cytotoxicity is most likely due to cellular recognition by the N-terminal extension, since the intrinsic activity of the enzyme is not sufficient for cytotoxicity and the N-terminal extension does not affect the conformation of EDN.
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==Disease==
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Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities.,Chang C, Newton DL, Rybak SM, Wlodawer A J Mol Biol. 2002 Mar 15;317(1):119-30. PMID:11916383<ref>PMID:11916383</ref>
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Known diseases associated with this structure: Central hypoventilation syndrome, congenital OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131242 131242]], Hirschsprung disease OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131242 131242]], Shah-Waardenburg syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131242 131242]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1K2A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K2A OCA].
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</div>
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<div class="pdbe-citations 1k2a" style="background-color:#fffaf0;"></div>
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==Reference==
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== References ==
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Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities., Chang C, Newton DL, Rybak SM, Wlodawer A, J Mol Biol. 2002 Mar 15;317(1):119-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11916383 11916383]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Chang C]]
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[[Category: Chang, C.]]
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[[Category: Newton DL]]
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[[Category: Newton, D.L.]]
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[[Category: Rybak SM]]
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[[Category: Rybak, S.M.]]
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[[Category: Wlodawer A]]
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[[Category: Wlodawer, A.]]
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[[Category: SO4]]
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[[Category: rnase a folding]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:46:47 2007''
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Current revision

Modified Form of Eosinophil-derived Neurotoxin

PDB ID 1k2a

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