1tqh

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(New page: 200px<br /><applet load="1tqh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tqh, resolution 1.63&Aring;" /> '''Covalent Reaction in...)
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[[Image:1tqh.jpg|left|200px]]<br /><applet load="1tqh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tqh, resolution 1.63&Aring;" />
 
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'''Covalent Reaction intermediate Revealed in Crystal Structure of the Geobacillus stearothermophilus Carboxylesterase Est30'''<br />
 
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==Overview==
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==Covalent Reaction intermediate Revealed in Crystal Structure of the Geobacillus stearothermophilus Carboxylesterase Est30==
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Est30 is a thermophilic carboxylesterase cloned from Geobacillus, stearothermophilus that showed optimal hydrolysis of esters with short, acyl chains at 70 degrees C. Est30 is a member of a new family of, carboxylesterases with representatives in other Gram-positive bacteria., The crystal structure has been determined at 1.63A resolution using, multiple anomalous dispersion data. The two-domain crystal structure, showed a large domain with a modified alpha/beta hydrolase core including, a seven, rather than an eight-stranded beta sheet, and a smaller cap, domain comprising three alpha helices. The catalytic triad consists of, residues Ser94, Asp193, and His223. A 100Da tetrahedral ligand was, observed to be covalently bound to the side-chain of Ser94. The propyl, acetate ligand represents the first tetrahedral intermediate in the, reaction mechanism. Therefore, this Est30 crystal structure will help, understand the mode of action of all enzymes in the serine hydrolase, superfamily.
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<StructureSection load='1tqh' size='340' side='right'caption='[[1tqh]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tqh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TQH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4PA:PROPYL+ACETATE'>4PA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tqh OCA], [https://pdbe.org/1tqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tqh RCSB], [https://www.ebi.ac.uk/pdbsum/1tqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tqh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EST_GEOSE EST_GEOSE] Involved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tq/1tqh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tqh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Est30 is a thermophilic carboxylesterase cloned from Geobacillus stearothermophilus that showed optimal hydrolysis of esters with short acyl chains at 70 degrees C. Est30 is a member of a new family of carboxylesterases with representatives in other Gram-positive bacteria. The crystal structure has been determined at 1.63A resolution using multiple anomalous dispersion data. The two-domain crystal structure showed a large domain with a modified alpha/beta hydrolase core including a seven, rather than an eight-stranded beta sheet, and a smaller cap domain comprising three alpha helices. The catalytic triad consists of residues Ser94, Asp193, and His223. A 100Da tetrahedral ligand was observed to be covalently bound to the side-chain of Ser94. The propyl acetate ligand represents the first tetrahedral intermediate in the reaction mechanism. Therefore, this Est30 crystal structure will help understand the mode of action of all enzymes in the serine hydrolase superfamily.
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==About this Structure==
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Covalent reaction intermediate revealed in crystal structure of the Geobacillus stearothermophilus carboxylesterase Est30.,Liu P, Wang YF, Ewis HE, Abdelal AT, Lu CD, Harrison RW, Weber IT J Mol Biol. 2004 Sep 10;342(2):551-61. PMID:15327954<ref>PMID:15327954</ref>
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1TQH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with SO4 and 4PA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TQH OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Covalent reaction intermediate revealed in crystal structure of the Geobacillus stearothermophilus carboxylesterase Est30., Liu P, Wang YF, Ewis HE, Abdelal AT, Lu CD, Harrison RW, Weber IT, J Mol Biol. 2004 Sep 10;342(2):551-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15327954 15327954]
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</div>
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[[Category: Carboxylesterase]]
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<div class="pdbe-citations 1tqh" style="background-color:#fffaf0;"></div>
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[[Category: Geobacillus stearothermophilus]]
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[[Category: Single protein]]
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[[Category: Abdelal, A.T.]]
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[[Category: Ewis, H.E.]]
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[[Category: Harrison, R.W.]]
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[[Category: Liu, P.]]
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[[Category: Lu, C.D.]]
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[[Category: Wang, Y.F.]]
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[[Category: Weber, I.T.]]
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[[Category: 4PA]]
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[[Category: SO4]]
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[[Category: alpha/beta hydrolase]]
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[[Category: carboxylesterase]]
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[[Category: tetrahedral intermediate]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:30:48 2007''
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==See Also==
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*[[Carboxylesterase 3D structures|Carboxylesterase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Geobacillus stearothermophilus]]
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[[Category: Large Structures]]
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[[Category: Abdelal AT]]
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[[Category: Ewis HE]]
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[[Category: Harrison RW]]
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[[Category: Liu P]]
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[[Category: Lu CD]]
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[[Category: Wang YF]]
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[[Category: Weber IT]]

Current revision

Covalent Reaction intermediate Revealed in Crystal Structure of the Geobacillus stearothermophilus Carboxylesterase Est30

PDB ID 1tqh

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