1uv4

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{{Seed}}
 
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[[Image:1uv4.png|left|200px]]
 
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==Native Bacillus subtilis Arabinanase Arb43A==
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The line below this paragraph, containing "STRUCTURE_1uv4", creates the "Structure Box" on the page.
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<StructureSection load='1uv4' size='340' side='right'caption='[[1uv4]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1uv4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UV4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UV4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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{{STRUCTURE_1uv4| PDB=1uv4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uv4 OCA], [https://pdbe.org/1uv4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uv4 RCSB], [https://www.ebi.ac.uk/pdbsum/1uv4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uv4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EABN1_BACSU EABN1_BACSU] Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the internal cleavage of alpha-(1->5)-L-arabinofuranosyl residues of linear 1,5-alpha-L-arabinan and of branched sugar beet arabinan. It displays no activity against heavily substituted arabinans or a range of other polysaccharides (larch wood arabinogalactan, wheat arabinoxylan and p-nitrophenyl-alpha-L-arabinofuranoside). The enzyme activity is progressively reduced as alpha-(1->5)-chains become shorter or more highly substituted.<ref>PMID:14973026</ref> <ref>PMID:15556708</ref> <ref>PMID:15708971</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uv/1uv4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uv4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Enzymes acting on polymeric substrates are frequently classified as exo or endo, reflecting their preference for, or ignorance of, polymer chain ends. Most biotechnological applications, especially in the field of polysaccharide degradation, require either endo- or exo-acting hydrolases, or they harness the essential synergy between these two modes of action. Here, we have used genomic data in tandem with structure to modify, radically, the chain-end specificity of the Cellvibrio japonicus exo-arabinanase CjArb43A. The structure of Bacillus subtilis endo-arabinanase 43A (BsArb43A) in harness with chain-end recognition kinetics of CjArb43A directed a rational design approach that led to the conversion of the Cellvibrio enzyme from an exo to an endo mode of action. One of the exo-acting mutants, D35L/Q316A, displays similar activity to WT CjArb43A and the removal of the steric block mediated by the side chains of Gln-316 and Asp-53 at the -3 subsite confers its capacity to attack internal glycoside bonds. This study provides a template for the production of tailored industrial catalysts. The introduction of subtle changes informed by comparative 3D structural and genomic data can lead to fundamental changes in the mode of action of these enzymes.
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===NATIVE BACILLUS SUBTILIS ARABINANASE ARB43A===
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Tailored catalysts for plant cell-wall degradation: redesigning the exo/endo preference of Cellvibrio japonicus arabinanase 43A.,Proctor MR, Taylor EJ, Nurizzo D, Turkenburg JP, Lloyd RM, Vardakou M, Davies GJ, Gilbert HJ Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):2697-702. Epub 2005 Feb 11. PMID:15708971<ref>PMID:15708971</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1uv4" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15708971}}, adds the Publication Abstract to the page
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*[[Arabinanase 3D structures|Arabinanase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15708971 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15708971}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1UV4 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UV4 OCA].
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==Reference==
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<ref group="xtra">PMID:15708971</ref><references group="xtra"/>
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[[Category: Arabinan endo-1,5-alpha-L-arabinosidase]]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Davies, G J.]]
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[[Category: Large Structures]]
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[[Category: Gilbert, H J.]]
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[[Category: Davies GJ]]
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[[Category: Nurizzo, D.]]
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[[Category: Gilbert HJ]]
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[[Category: Taylor, E J.]]
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[[Category: Nurizzo D]]
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[[Category: Arabinase]]
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[[Category: Taylor EJ]]
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[[Category: Bacillus]]
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[[Category: Catalysis]]
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[[Category: Hydrolase]]
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[[Category: Propeller]]
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[[Category: Subtili]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 01:08:31 2009''
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Current revision

Native Bacillus subtilis Arabinanase Arb43A

PDB ID 1uv4

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