1v91

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[[Image:1v91.gif|left|200px]]
 
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==Solution structure of insectidal toxin delta-paluIT2-NH2==
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The line below this paragraph, containing "STRUCTURE_1v91", creates the "Structure Box" on the page.
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<StructureSection load='1v91' size='340' side='right'caption='[[1v91]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1v91]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pireneitega_luctuosa Pireneitega luctuosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V91 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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{{STRUCTURE_1v91| PDB=1v91 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v91 OCA], [https://pdbe.org/1v91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v91 RCSB], [https://www.ebi.ac.uk/pdbsum/1v91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v91 ProSAT]</span></td></tr>
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</table>
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'''Solution structure of insectidal toxin delta-paluIT2-NH2'''
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== Function ==
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[https://www.uniprot.org/uniprot/T3D1B_PIRLC T3D1B_PIRLC] Insecticidal toxin. Binds to site 4 of insect voltage-gated sodium channel (Nav) and inhibits channel inactivation. In vivo, it lethal to lepidopteran larvae. Has no adverse affects when intracerebroventricularly injected in mice at a dose of 0.2 ug, but causes reversible paralysis of legs when injected intracerebroventricularly in mice at a dose of 2.0 ug.<ref>PMID:10971590</ref> <ref>PMID:15683238</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/1v91_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v91 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Delta-paluIT1 and delta-paluIT2 are toxins purified from the venom of the spider Paracoelotes luctuosus. Similar in sequence to mu-agatoxins from Agelenopsis aperta, their pharmacological target is the voltage-gated insect sodium channel, of which they alter the inactivation properties in a way similar to alpha-scorpion toxins, but they bind on site 4 in a way similar to beta-scorpion toxins. We determined the solution structure of the two toxins by use of two-dimensional nuclear magnetic resonance (NMR) techniques followed by distance geometry and molecular dynamics. The structures of delta-paluIT1 and delta-paluIT2 belong to the inhibitory cystine knot structural family, i.e. a compact disulfide-bonded core from which four loops emerge. Delta-paluIT1 and delta-paluIT2 contain respectively two- and three-stranded anti-parallel beta-sheets as unique secondary structure. We compare the structure and the electrostatic anisotropy of those peptides to other sodium and calcium channel toxins, analyze the topological juxtaposition of key functional residues, and conclude that the recognition of insect voltage-gated sodium channels by these toxins involves the beta-sheet, in addition to loops I and IV. Besides the position of culprit residues on the molecular surface, difference in dipolar moment orientation is another determinant of receptor binding and biological activity differences. We also demonstrate by electrophysiological experiments on the cloned insect voltage-gated sodium channel, para, heterologuously co-expressed with the tipE subunit in Xenopus laevis oocytes, that delta-paluIT1 and delta-paluIT2 procure an increase of Na+ current. delta-PaluIT1-OH seems to have less effect when the same concentrations are used.
Delta-paluIT1 and delta-paluIT2 are toxins purified from the venom of the spider Paracoelotes luctuosus. Similar in sequence to mu-agatoxins from Agelenopsis aperta, their pharmacological target is the voltage-gated insect sodium channel, of which they alter the inactivation properties in a way similar to alpha-scorpion toxins, but they bind on site 4 in a way similar to beta-scorpion toxins. We determined the solution structure of the two toxins by use of two-dimensional nuclear magnetic resonance (NMR) techniques followed by distance geometry and molecular dynamics. The structures of delta-paluIT1 and delta-paluIT2 belong to the inhibitory cystine knot structural family, i.e. a compact disulfide-bonded core from which four loops emerge. Delta-paluIT1 and delta-paluIT2 contain respectively two- and three-stranded anti-parallel beta-sheets as unique secondary structure. We compare the structure and the electrostatic anisotropy of those peptides to other sodium and calcium channel toxins, analyze the topological juxtaposition of key functional residues, and conclude that the recognition of insect voltage-gated sodium channels by these toxins involves the beta-sheet, in addition to loops I and IV. Besides the position of culprit residues on the molecular surface, difference in dipolar moment orientation is another determinant of receptor binding and biological activity differences. We also demonstrate by electrophysiological experiments on the cloned insect voltage-gated sodium channel, para, heterologuously co-expressed with the tipE subunit in Xenopus laevis oocytes, that delta-paluIT1 and delta-paluIT2 procure an increase of Na+ current. delta-PaluIT1-OH seems to have less effect when the same concentrations are used.
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==About this Structure==
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Solution structure of two insect-specific spider toxins and their pharmacological interaction with the insect voltage-gated Na+ channel.,Ferrat G, Bosmans F, Tytgat J, Pimentel C, Chagot B, Gilles N, Nakajima T, Darbon H, Corzo G Proteins. 2005 May 1;59(2):368-79. PMID:15726637<ref>PMID:15726637</ref>
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1V91 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paracoelotes_luctuosus Paracoelotes luctuosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V91 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of two insect-specific spider toxins and their pharmacological interaction with the insect voltage-gated Na+ channel., Ferrat G, Bosmans F, Tytgat J, Pimentel C, Chagot B, Gilles N, Nakajima T, Darbon H, Corzo G, Proteins. 2005 May 1;59(2):368-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15726637 15726637]
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</div>
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[[Category: Paracoelotes luctuosus]]
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<div class="pdbe-citations 1v91" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Bosmans, F.]]
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<references/>
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[[Category: Chagot, B.]]
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__TOC__
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[[Category: Corzo, G.]]
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</StructureSection>
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[[Category: Darbon, H.]]
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[[Category: Large Structures]]
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[[Category: Ferrat, G.]]
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[[Category: Pireneitega luctuosa]]
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[[Category: Nakajima, T.]]
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[[Category: Bosmans F]]
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[[Category: Pimentel, C.]]
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[[Category: Chagot B]]
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[[Category: Tytgat, J.]]
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[[Category: Corzo G]]
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[[Category: Amidation]]
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[[Category: Darbon H]]
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[[Category: Ionic channel inhibitor]]
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[[Category: Ferrat G]]
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[[Category: Neurotoxin]]
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[[Category: Nakajima T]]
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[[Category: Sodium channel inhibitor]]
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[[Category: Pimentel C]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:14:35 2008''
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[[Category: Tytgat J]]

Current revision

Solution structure of insectidal toxin delta-paluIT2-NH2

PDB ID 1v91

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