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1u78
From Proteopedia
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[[Image:1u78.gif|left|200px]] | [[Image:1u78.gif|left|200px]] | ||
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'''Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA''' | '''Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA''' | ||
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[[Category: Sixma, T K.]] | [[Category: Sixma, T K.]] | ||
[[Category: Watkins, S.]] | [[Category: Watkins, S.]] | ||
| - | [[Category: | + | [[Category: Bipartite dna-binding]] |
| - | [[Category: | + | [[Category: Hth-motif]] |
| - | [[Category: | + | [[Category: Transposase]] |
| - | [[Category: | + | [[Category: Transposon dna]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:50:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:50, 3 May 2008
Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA
Overview
The bipartite DNA-binding domain of Tc3 transposase, Tc3A, was crystallized in complex with its transposon recognition sequence. In the structure the two DNA-binding domains form structurally related helix-turn-helix (HTH) motifs. They both bind to the major groove on a single DNA oligomer, separated by a linker that interacts closely with the minor groove. The structure resembles that of the transcription factor Pax6 DNA-binding domain, but the relative orientation of the HTH-domain is different. The DNA conformation is distorted, characterized by local narrowing of the minor groove and bends at both ends. The protein-DNA recognition takes place through base and backbone contacts, as well as shape-recognition of the distortions in the DNA. Charged interactions are primarily found in the N-terminal domain and the linker indicating that these may form the initial contact area. Two independent dimer interfaces could be relevant for bringing together transposon ends and for binding to a direct repeat site in the transposon end. In contrast to the Tn5 synaptic complex, the two Tc3A DNA-binding domains bind to a single Tc3 transposon end.
About this Structure
1U78 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Structural analysis of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA., Watkins S, van Pouderoyen G, Sixma TK, Nucleic Acids Res. 2004 Aug 10;32(14):4306-12. Print 2004. PMID:15304566 Page seeded by OCA on Sat May 3 10:50:29 2008
