1aiw

From Proteopedia

(Difference between revisions)

Current revision

NMR STRUCTURES OF THE CELLULOSE-BINDING DOMAIN OF THE ENDOGLUCANASE Z FROM ERWINIA CHRYSANTHEMI, 23 STRUCTURES

Structural highlights

1aiw is a 1 chain structure with sequence from Dickeya dadantii 3937. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 23 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GUNZ_DICD3 Represents 97% of the global cellulase activity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Two-dimensional proton nuclear magnetic resonance spectroscopy has been used to determine the three-dimensional structure of the 62 amino acid C-terminal cellulose-binding domain (CBD) of the endoglucanase Z (CBDEGZ), secreted by Erwinia chrysanthemi. An experimental data set comprising 958 interproton nOe-derived restraints was used to calculate 23 structures. The calculated structures have an average root-mean-square deviation between Cys4 and Cys61 of 0.91 +/- 0.11 A for backbone atoms and 1.18 +/- 0.12 A for the heavy atoms. The CBDEGZ exhibits a skiboot shape based mainly on a triple antiparallel beta-sheet perpendicular to a less-ordered summital loop. Three aromatic rings (Trp18, Trp43, and Tyr44) are localized on one face of the protein and are exposed to the solvent in a conformation compatible with a cellulose-binding site. Based on its original folding, we have been able to relate the CBD sequence to those of several domains of unknown function occurring in several bacterial chitinases as well as other proteins. This study also provides a structural basis for analyzing the secretion-related information specific to the CBDEGZ.

Solution structure of the cellulose-binding domain of the endoglucanase Z secreted by Erwinia chrysanthemi.,Brun E, Moriaud F, Gans P, Blackledge MJ, Barras F, Marion D Biochemistry. 1997 Dec 23;36(51):16074-86. PMID:9405041^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Brun E, Moriaud F, Gans P, Blackledge MJ, Barras F, Marion D. Solution structure of the cellulose-binding domain of the endoglucanase Z secreted by Erwinia chrysanthemi. Biochemistry. 1997 Dec 23;36(51):16074-86. PMID:9405041 doi:10.1021/bi9718494

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1aiw"

@@ Line 1: / Line 1: @@
 ==NMR STRUCTURES OF THE CELLULOSE-BINDING DOMAIN OF THE ENDOGLUCANASE Z FROM ERWINIA CHRYSANTHEMI, 23 STRUCTURES==
-<StructureSection load='1aiw' size='340' side='right' caption='[[1aiw]], [[NMR_Ensembles_of_Models | 23 NMR models]]' scene=''>
+<StructureSection load='1aiw' size='340' side='right'caption='[[1aiw]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1aiw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AIW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1aiw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AIW FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CELZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 23 models</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aiw OCA], [https://pdbe.org/1aiw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aiw RCSB], [https://www.ebi.ac.uk/pdbsum/1aiw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aiw ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aiw OCA], [http://pdbe.org/1aiw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1aiw RCSB], [http://www.ebi.ac.uk/pdbsum/1aiw PDBsum]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GUNZ_DICD3 GUNZ_DICD3]] Represents 97% of the global cellulase activity.
+[https://www.uniprot.org/uniprot/GUNZ_DICD3 GUNZ_DICD3] Represents 97% of the global cellulase activity.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/1aiw_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/1aiw_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Glucanase|Glucanase]]
+*[[Glucanase 3D structures|Glucanase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Cellulase]]
+[[Category: Dickeya dadantii 3937]]
-[[Category: Dicd3]]
+[[Category: Large Structures]]
-[[Category: Barras, F]]
+[[Category: Barras F]]
-[[Category: Blackledge, M J]]
+[[Category: Blackledge MJ]]
-[[Category: Brun, E]]
+[[Category: Brun E]]
-[[Category: Gans, P]]
+[[Category: Gans P]]
-[[Category: Marion, D]]
+[[Category: Marion D]]
-[[Category: Moriaud, F]]
+[[Category: Moriaud F]]
-[[Category: Cellulose degradation]]
-[[Category: Cellulose-binding domain]]
-[[Category: Endoglucanase]]
-[[Category: Erwinia chrysanthemi]]

1aiw

From Proteopedia

Current revision

NMR STRUCTURES OF THE CELLULOSE-BINDING DOMAIN OF THE ENDOGLUCANASE Z FROM ERWINIA CHRYSANTHEMI, 23 STRUCTURES

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox