1h5d

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (00:02, 21 November 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1h5d.jpg|left|200px]]
 
-
{{Structure
+
==X-ray induced reduction of horseradish peroxidase C1A Compound III (0-11% dose)==
-
|PDB= 1h5d |SIZE=350|CAPTION= <scene name='initialview01'>1h5d</scene>, resolution 1.60&Aring;
+
<StructureSection load='1h5d' size='340' side='right'caption='[[1h5d]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
-
|SITE= <scene name='pdbsite=HEM:Hem+Binding+Site+For+Chain+A'>HEM</scene>
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
+
<table><tr><td colspan='2'>[[1h5d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H5D FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h5d OCA], [https://pdbe.org/1h5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h5d RCSB], [https://www.ebi.ac.uk/pdbsum/1h5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h5d ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h5d OCA], [http://www.ebi.ac.uk/pdbsum/1h5d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h5d RCSB]</span>
+
== Evolutionary Conservation ==
-
}}
+
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h5/1h5d_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h5d ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.
-
'''X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (0-11% DOSE)'''
+
The catalytic pathway of horseradish peroxidase at high resolution.,Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J Nature. 2002 May 23;417(6887):463-8. PMID:12024218<ref>PMID:12024218</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1h5d" style="background-color:#fffaf0;"></div>
-
==Overview==
+
==See Also==
-
A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.
+
*[[Horseradish peroxidase|Horseradish peroxidase]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
1H5D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5D OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
-
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12024218 12024218]
+
[[Category: Armoracia rusticana]]
[[Category: Armoracia rusticana]]
-
[[Category: Peroxidase]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Berglund GI]]
-
[[Category: Berglund, G I.]]
+
[[Category: Carlsson GH]]
-
[[Category: Carlsson, G H.]]
+
[[Category: Hajdu J]]
-
[[Category: Hajdu, J.]]
+
[[Category: Henriksen A]]
-
[[Category: Henriksen, A.]]
+
[[Category: Smith AT]]
-
[[Category: Smith, A T.]]
+
[[Category: Szoke H]]
-
[[Category: Szoke, H.]]
+
-
[[Category: compound iii]]
+
-
[[Category: horseradish]]
+
-
[[Category: oxidoreductase]]
+
-
[[Category: oxyperoxidase]]
+
-
[[Category: peroxidase]]
+
-
[[Category: x-ray induced reduction]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:58:05 2008''
+

Current revision

X-ray induced reduction of horseradish peroxidase C1A Compound III (0-11% dose)

PDB ID 1h5d

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1h5d"
Personal tools