1ixc

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[[Image:1ixc.gif|left|200px]]
 
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==Crystal structure of CbnR, a LysR family transcriptional regulator==
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The line below this paragraph, containing "STRUCTURE_1ixc", creates the "Structure Box" on the page.
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<StructureSection load='1ixc' size='340' side='right'caption='[[1ixc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ixc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_necator Cupriavidus necator]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IXC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1ixc| PDB=1ixc | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ixc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ixc OCA], [https://pdbe.org/1ixc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ixc RCSB], [https://www.ebi.ac.uk/pdbsum/1ixc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ixc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9WXC7_CUPNE Q9WXC7_CUPNE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/1ixc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ixc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The LysR-type transcriptional regulator (LTTR) proteins are one of the most common transcriptional regulators in prokaryotes. Here we report the crystal structure of CbnR, which is one of the LTTRs derived from Ralstonia eutropha NH9. This is the first crystal structure of a full-length LTTR. CbnR was found to form a homo-tetramer, which seems to be a biologically active form. Surprisingly, the tetramer can be regarded as a dimer of dimers, whereby each dimer is composed of two subunits in different conformations. In the CbnR tetramer, the DNA-binding domains are located at the V-shaped bottom of the main body of the tetramer, and seem to be suitable to interact with a long stretch of the promoter DNA, which is approximately 60bp. Interaction between the four DNA-binding domains and the two binding sites on the target DNA is likely to bend the target DNA along the V-shaped bottom of the CbnR tetramer. The relaxation of the bent DNA, which occurs upon inducer binding to CbnR, seems to be associated with a quaternary structure change of the tetramer.
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'''Crystal structure of CbnR, a LysR family transcriptional regulator'''
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Crystal structure of a full-length LysR-type transcriptional regulator, CbnR: unusual combination of two subunit forms and molecular bases for causing and changing DNA bend.,Muraoka S, Okumura R, Ogawa N, Nonaka T, Miyashita K, Senda T J Mol Biol. 2003 May 2;328(3):555-66. PMID:12706716<ref>PMID:12706716</ref>
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==Overview==
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The LysR-type transcriptional regulator (LTTR) proteins are one of the most common transcriptional regulators in prokaryotes. Here we report the crystal structure of CbnR, which is one of the LTTRs derived from Ralstonia eutropha NH9. This is the first crystal structure of a full-length LTTR. CbnR was found to form a homo-tetramer, which seems to be a biologically active form. Surprisingly, the tetramer can be regarded as a dimer of dimers, whereby each dimer is composed of two subunits in different conformations. In the CbnR tetramer, the DNA-binding domains are located at the V-shaped bottom of the main body of the tetramer, and seem to be suitable to interact with a long stretch of the promoter DNA, which is approximately 60bp. Interaction between the four DNA-binding domains and the two binding sites on the target DNA is likely to bend the target DNA along the V-shaped bottom of the CbnR tetramer. The relaxation of the bent DNA, which occurs upon inducer binding to CbnR, seems to be associated with a quaternary structure change of the tetramer.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1IXC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cupriavidus_necator Cupriavidus necator]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXC OCA].
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</div>
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<div class="pdbe-citations 1ixc" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Crystal structure of a full-length LysR-type transcriptional regulator, CbnR: unusual combination of two subunit forms and molecular bases for causing and changing DNA bend., Muraoka S, Okumura R, Ogawa N, Nonaka T, Miyashita K, Senda T, J Mol Biol. 2003 May 2;328(3):555-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12706716 12706716]
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Cupriavidus necator]]
[[Category: Cupriavidus necator]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Miyashita, K.]]
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[[Category: Miyashita K]]
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[[Category: Muraoka, S.]]
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[[Category: Muraoka S]]
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[[Category: Ogawa, N.]]
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[[Category: Ogawa N]]
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[[Category: Okumura, R.]]
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[[Category: Okumura R]]
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[[Category: Senda, T.]]
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[[Category: Senda T]]
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[[Category: Long alpha helix connecting dna binding and regulatory domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:32:21 2008''
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Current revision

Crystal structure of CbnR, a LysR family transcriptional regulator

PDB ID 1ixc

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