1jnj

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[[Image:1jnj.jpg|left|200px]]<br /><applet load="1jnj" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jnj" />
 
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'''NMR solution structure of the human beta2-microglobulin'''<br />
 
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==Overview==
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==NMR solution structure of the human beta2-microglobulin==
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The solution structure of human beta2-microglobulin (beta2-m), the, nonpolymorphic component of class I major histocompatibility complex, (MHC-I), was determined by (1)H NMR spectroscopy and restrained modeling, calculations. Compared to previous structural data obtained from the NMR, secondary structure of the isolated protein and the crystal structure of, MHC-I, in which the protein is associated to the heavy-chain component, several differences are observed. The most important rearrangements were, observed for (1) strands V and VI (loss of the C-terminal and N-terminal, end, respectively), (2) interstrand loop V-VI, and (3) strand I, including, the N-terminal segment (displacement outward of the molecular core). These, modifications can be considered as the prodromes of the amyloid, transition. Solvation of the protected regions in MHC-I decreases the, tertiary packing by breaking the contiguity of the surface hydrophobic, patches at the interface with heavy chain and the nearby region at the, surface charge cluster of the C-terminal segment. As a result, the, molecule is placed in a state in which even minor charge and solvation, changes in response to pH or ionic-strength variations can easily, compromise the hydrophobic/hydrophilic balance and trigger the transition, into a partially unfolded intermediate that starts with unpairing of, strand I and leads to polymerization and precipitation into fibrils or, amorphous aggregates. The same mechanism accounts for the partial, unfolding and fiber formation subsequent to Cu(2+) binding, which is shown, to occur primarily at His 31 and involve partially also His 13, the next, available His residue along the partial unfolding pathway.
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<StructureSection load='1jnj' size='340' side='right'caption='[[1jnj]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jnj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JNJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jnj OCA], [https://pdbe.org/1jnj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jnj RCSB], [https://www.ebi.ac.uk/pdbsum/1jnj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jnj ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/B2MG_HUMAN B2MG_HUMAN] Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:[https://omim.org/entry/241600 241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.<ref>PMID:16549777</ref> Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.<ref>PMID:3532124</ref> <ref>PMID:1336137</ref> <ref>PMID:7554280</ref> <ref>PMID:4586824</ref> <ref>PMID:8084451</ref> <ref>PMID:12119416</ref> <ref>PMID:12796775</ref> <ref>PMID:16901902</ref> <ref>PMID:16491088</ref> <ref>PMID:17646174</ref> <ref>PMID:18835253</ref> <ref>PMID:18395224</ref> <ref>PMID:19284997</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/B2MG_HUMAN B2MG_HUMAN] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/1jnj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jnj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The solution structure of human beta2-microglobulin (beta2-m), the nonpolymorphic component of class I major histocompatibility complex (MHC-I), was determined by (1)H NMR spectroscopy and restrained modeling calculations. Compared to previous structural data obtained from the NMR secondary structure of the isolated protein and the crystal structure of MHC-I, in which the protein is associated to the heavy-chain component, several differences are observed. The most important rearrangements were observed for (1) strands V and VI (loss of the C-terminal and N-terminal end, respectively), (2) interstrand loop V-VI, and (3) strand I, including the N-terminal segment (displacement outward of the molecular core). These modifications can be considered as the prodromes of the amyloid transition. Solvation of the protected regions in MHC-I decreases the tertiary packing by breaking the contiguity of the surface hydrophobic patches at the interface with heavy chain and the nearby region at the surface charge cluster of the C-terminal segment. As a result, the molecule is placed in a state in which even minor charge and solvation changes in response to pH or ionic-strength variations can easily compromise the hydrophobic/hydrophilic balance and trigger the transition into a partially unfolded intermediate that starts with unpairing of strand I and leads to polymerization and precipitation into fibrils or amorphous aggregates. The same mechanism accounts for the partial unfolding and fiber formation subsequent to Cu(2+) binding, which is shown to occur primarily at His 31 and involve partially also His 13, the next available His residue along the partial unfolding pathway.
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==Disease==
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The solution structure of human beta2-microglobulin reveals the prodromes of its amyloid transition.,Verdone G, Corazza A, Viglino P, Pettirossi F, Giorgetti S, Mangione P, Andreola A, Stoppini M, Bellotti V, Esposito G Protein Sci. 2002 Mar;11(3):487-99. PMID:11847272<ref>PMID:11847272</ref>
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Known disease associated with this structure: Hypoproteinemia, hypercatabolic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=109700 109700]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1JNJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNJ OCA].
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</div>
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<div class="pdbe-citations 1jnj" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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The solution structure of human beta2-microglobulin reveals the prodromes of its amyloid transition., Verdone G, Corazza A, Viglino P, Pettirossi F, Giorgetti S, Mangione P, Andreola A, Stoppini M, Bellotti V, Esposito G, Protein Sci. 2002 Mar;11(3):487-99. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11847272 11847272]
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*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Andreola, A.]]
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[[Category: Andreola A]]
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[[Category: Bellotti, V.]]
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[[Category: Bellotti V]]
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[[Category: Corazza, A.]]
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[[Category: Corazza A]]
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[[Category: Esposito, G.]]
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[[Category: Esposito G]]
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[[Category: Giorgetti, S.]]
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[[Category: Giorgetti S]]
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[[Category: Mangione, P.]]
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[[Category: Mangione P]]
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[[Category: Pettirossi, F.]]
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[[Category: Pettirossi F]]
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[[Category: Stoppini, M.]]
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[[Category: Stoppini M]]
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[[Category: Verdone, G.]]
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[[Category: Verdone G]]
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[[Category: Viglino, P.]]
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[[Category: Viglino P]]
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[[Category: immunoglobulin constant domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:08:12 2008''
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Current revision

NMR solution structure of the human beta2-microglobulin

PDB ID 1jnj

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