1l1m

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{{Seed}}
 
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[[Image:1l1m.png|left|200px]]
 
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==SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O1==
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The line below this paragraph, containing "STRUCTURE_1l1m", creates the "Structure Box" on the page.
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<StructureSection load='1l1m' size='340' side='right'caption='[[1l1m]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1l1m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L1M FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l1m OCA], [https://pdbe.org/1l1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l1m RCSB], [https://www.ebi.ac.uk/pdbsum/1l1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l1m ProSAT]</span></td></tr>
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{{STRUCTURE_1l1m| PDB=1l1m | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l1/1l1m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l1m ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The lac repressor-operator system is a model system for understanding protein-DNA interactions and allosteric mechanisms in gene regulation. Despite the wealth of biochemical data provided by extensive mutations of both repressor and operator, the specific recognition mechanism of the natural lac operators by lac repressor has remained elusive. Here we present the first high-resolution structure of a dimer of the DNA-binding domain of lac repressor bound to its natural operator 01. The global positioning of the dimer on the operator is dramatically asymmetric, which results in a different pattern of specific contacts between the two sites. Specific recognition is accomplished by a combination of elongation and twist by 48 degrees of the right lac subunit relative to the left one, significant rearrangement of many side chains as well as sequence-dependent deformability of the DNA. The set of recognition mechanisms involved in the lac repressor-operator system is unique among other protein-DNA complexes and presents a nice example of the adaptability that both proteins and DNA exhibit in the context of their mutual interaction.
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===SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O1===
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Plasticity in protein-DNA recognition: lac repressor interacts with its natural operator 01 through alternative conformations of its DNA-binding domain.,Kalodimos CG, Bonvin AM, Salinas RK, Wechselberger R, Boelens R, Kaptein R EMBO J. 2002 Jun 17;21(12):2866-76. PMID:12065400<ref>PMID:12065400</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1l1m" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12065400}}, adds the Publication Abstract to the page
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*[[Lac repressor|Lac repressor]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12065400 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12065400}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1L1M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1M OCA].
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==Reference==
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Plasticity in protein-DNA recognition: lac repressor interacts with its natural operator 01 through alternative conformations of its DNA-binding domain., Kalodimos CG, Bonvin AM, Salinas RK, Wechselberger R, Boelens R, Kaptein R, EMBO J. 2002 Jun 17;21(12):2866-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12065400 12065400]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Boelens, R.]]
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[[Category: Boelens R]]
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[[Category: Bonvin, A M.J J.]]
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[[Category: Bonvin AMJJ]]
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[[Category: Kalodimos, C G.]]
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[[Category: Kalodimos CG]]
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[[Category: Kaptein, R.]]
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[[Category: Kaptein R]]
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[[Category: Salinas, R K.]]
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[[Category: Salinas RK]]
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[[Category: Wechselberger, R.]]
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[[Category: Wechselberger R]]
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[[Category: Asymmetric dna-binding]]
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[[Category: Hth]]
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[[Category: Lac operon]]
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[[Category: Lac repressor]]
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[[Category: Natural lac operator]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:29:54 2008''
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Current revision

SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O1

PDB ID 1l1m

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