1mk8

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(New page: 200px<br /><applet load="1mk8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mk8, resolution 1.65&Aring;" /> '''Crystal Structure of...)
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[[Image:1mk8.jpg|left|200px]]<br /><applet load="1mk8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mk8, resolution 1.65&Aring;" />
 
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'''Crystal Structure of a Mutant Cytochrome c Peroxidase showing a Novel Trp-Tyr Covalent Cross-link'''<br />
 
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==Overview==
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==Crystal Structure of a Mutant Cytochrome c Peroxidase showing a Novel Trp-Tyr Covalent Cross-link==
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The crystal structure of a cytochrome c peroxidase mutant where the distal, catalytic His52 is converted to Tyr reveals that the tyrosine side-chain, forms a covalent bond with the indole ring nitrogen atom of Trp51. We, hypothesize that this novel bond results from peroxide activation by the, heme iron followed by oxidation of Trp51 and Tyr52. This hypothesis has, been tested by incorporation of a redox-inactive Zn-protoporphyrin into, the protein, and the resulting crystal structure shows the absence of a, Trp51-Tyr52 cross-link. Instead, the Tyr52 side-chain orients away from, the heme active-site pocket, which requires a substantial rearrangement of, residues 72-80 and 134-144. Additional experiments where heme-containing, crystals of the mutant were treated with peroxide support our hypothesis, that this novel Trp-Tyr cross-link is a peroxide-dependent process, mediated by the heme iron.
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<StructureSection load='1mk8' size='340' side='right'caption='[[1mk8]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mk8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MK8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mk8 OCA], [https://pdbe.org/1mk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mk8 RCSB], [https://www.ebi.ac.uk/pdbsum/1mk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mk8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CCPR_YEAST CCPR_YEAST] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mk/1mk8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mk8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of a cytochrome c peroxidase mutant where the distal catalytic His52 is converted to Tyr reveals that the tyrosine side-chain forms a covalent bond with the indole ring nitrogen atom of Trp51. We hypothesize that this novel bond results from peroxide activation by the heme iron followed by oxidation of Trp51 and Tyr52. This hypothesis has been tested by incorporation of a redox-inactive Zn-protoporphyrin into the protein, and the resulting crystal structure shows the absence of a Trp51-Tyr52 cross-link. Instead, the Tyr52 side-chain orients away from the heme active-site pocket, which requires a substantial rearrangement of residues 72-80 and 134-144. Additional experiments where heme-containing crystals of the mutant were treated with peroxide support our hypothesis that this novel Trp-Tyr cross-link is a peroxide-dependent process mediated by the heme iron.
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==About this Structure==
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A novel heme and peroxide-dependent tryptophan-tyrosine cross-link in a mutant of cytochrome c peroxidase.,Bhaskar B, Immoos CE, Shimizu H, Sulc F, Farmer PJ, Poulos TL J Mol Biol. 2003 Apr 18;328(1):157-66. PMID:12684005<ref>PMID:12684005</ref>
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1MK8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cytochrome-c_peroxidase Cytochrome-c peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MK8 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A novel heme and peroxide-dependent tryptophan-tyrosine cross-link in a mutant of cytochrome c peroxidase., Bhaskar B, Immoos CE, Shimizu H, Sulc F, Farmer PJ, Poulos TL, J Mol Biol. 2003 Apr 18;328(1):157-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12684005 12684005]
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</div>
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[[Category: Cytochrome-c peroxidase]]
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<div class="pdbe-citations 1mk8" style="background-color:#fffaf0;"></div>
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Bhaskar, B.]]
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[[Category: Farmer, P.J.]]
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[[Category: Immoos, C.E.]]
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[[Category: Poulos, T.L.]]
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[[Category: Shimizu, H.]]
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[[Category: HEM]]
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[[Category: crystal structure]]
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[[Category: cytochrome c peroxidase]]
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[[Category: oxygen radical]]
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[[Category: trp cation radical]]
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[[Category: tryptophan-tyrosine cross-link]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:28:29 2007''
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==See Also==
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*[[Cytochrome c peroxidase 3D structures|Cytochrome c peroxidase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Bhaskar B]]
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[[Category: Farmer PJ]]
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[[Category: Immoos CE]]
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[[Category: Poulos TL]]
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[[Category: Shimizu H]]

Current revision

Crystal Structure of a Mutant Cytochrome c Peroxidase showing a Novel Trp-Tyr Covalent Cross-link

PDB ID 1mk8

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