1orq

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[[Image:1orq.gif|left|200px]]
 
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{{Structure
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==X-ray structure of a voltage-dependent potassium channel in complex with an Fab==
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|PDB= 1orq |SIZE=350|CAPTION= <scene name='initialview01'>1orq</scene>, resolution 3.20&Aring;
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<StructureSection load='1orq' size='340' side='right'caption='[[1orq]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>
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<table><tr><td colspan='2'>[[1orq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ORQ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1orq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1orq OCA], [https://pdbe.org/1orq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1orq RCSB], [https://www.ebi.ac.uk/pdbsum/1orq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1orq ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1ors|1ORS]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1orq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1orq OCA], [http://www.ebi.ac.uk/pdbsum/1orq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1orq RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/1orq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1orq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Voltage-dependent K+ channels are members of the family of voltage-dependent cation (K+, Na+ and Ca2+) channels that open and allow ion conduction in response to changes in cell membrane voltage. This form of gating underlies the generation of nerve and muscle action potentials, among other processes. Here we present the structure of KvAP, a voltage-dependent K+ channel from Aeropyrum pernix. We have determined a crystal structure of the full-length channel at a resolution of 3.2 A, and of the isolated voltage-sensor domain at 1.9 A, both in complex with monoclonal Fab fragments. The channel contains a central ion-conduction pore surrounded by voltage sensors, which form what we call 'voltage-sensor paddles'-hydrophobic, cationic, helix-turn-helix structures on the channel's outer perimeter. Flexible hinges suggest that the voltage-sensor paddles move in response to membrane voltage changes, carrying their positive charge across the membrane.
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'''X-ray structure of a voltage-dependent potassium channel in complex with an Fab'''
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X-ray structure of a voltage-dependent K+ channel.,Jiang Y, Lee A, Chen J, Ruta V, Cadene M, Chait BT, MacKinnon R Nature. 2003 May 1;423(6935):33-41. PMID:12721618<ref>PMID:12721618</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1orq" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Voltage-dependent K+ channels are members of the family of voltage-dependent cation (K+, Na+ and Ca2+) channels that open and allow ion conduction in response to changes in cell membrane voltage. This form of gating underlies the generation of nerve and muscle action potentials, among other processes. Here we present the structure of KvAP, a voltage-dependent K+ channel from Aeropyrum pernix. We have determined a crystal structure of the full-length channel at a resolution of 3.2 A, and of the isolated voltage-sensor domain at 1.9 A, both in complex with monoclonal Fab fragments. The channel contains a central ion-conduction pore surrounded by voltage sensors, which form what we call 'voltage-sensor paddles'-hydrophobic, cationic, helix-turn-helix structures on the channel's outer perimeter. Flexible hinges suggest that the voltage-sensor paddles move in response to membrane voltage changes, carrying their positive charge across the membrane.
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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==About this Structure==
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== References ==
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1ORQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORQ OCA].
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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X-ray structure of a voltage-dependent K+ channel., Jiang Y, Lee A, Chen J, Ruta V, Cadene M, Chait BT, MacKinnon R, Nature. 2003 May 1;423(6935):33-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12721618 12721618]
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[[Category: Aeropyrum pernix]]
[[Category: Aeropyrum pernix]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Cadene M]]
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[[Category: Cadene, M.]]
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[[Category: Chait BT]]
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[[Category: Chait, B T.]]
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[[Category: Chen J]]
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[[Category: Chen, J.]]
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[[Category: Jiang Y]]
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[[Category: Jiang, Y.]]
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[[Category: Lee A]]
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[[Category: Lee, A.]]
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[[Category: MacKinnon R]]
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[[Category: MacKinnon, R.]]
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[[Category: Ruta V]]
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[[Category: Ruta, V.]]
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[[Category: fab complex]]
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[[Category: kvap]]
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[[Category: potassium channel]]
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[[Category: voltage-dependent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:24 2008''
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Current revision

X-ray structure of a voltage-dependent potassium channel in complex with an Fab

PDB ID 1orq

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