1wuj

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[[Image:1wuj.png|left|200px]]
 
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==Three-Dimensional Structure Of The Ni-B State Of [Nife]Hydrogenase From Desulufovibrio Vulgaris Miyazaki F==
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The line below this paragraph, containing "STRUCTURE_1wuj", creates the "Structure Box" on the page.
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<StructureSection load='1wuj' size='340' side='right'caption='[[1wuj]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1wuj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._'Miyazaki_F' Desulfovibrio vulgaris str. 'Miyazaki F']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WUJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFO:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFO</scene></td></tr>
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{{STRUCTURE_1wuj| PDB=1wuj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wuj OCA], [https://pdbe.org/1wuj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wuj RCSB], [https://www.ebi.ac.uk/pdbsum/1wuj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wuj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHNS_NITV9 PHNS_NITV9] Catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wu/1wuj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wuj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hydrogenases catalyze oxidoreduction of molecular hydrogen and have potential applications for utilizing dihydrogen as an energy source. [NiFe] hydrogenase has two different oxidized states, Ni-A (unready, exhibits a lag phase in reductive activation) and Ni-B (ready). We have succeeded in converting Ni-B to Ni-A with the use of Na2S and O2 and determining the high-resolution crystal structures of both states. Ni-B possesses a monatomic nonprotein bridging ligand at the Ni-Fe active site, whereas Ni-A has a diatomic species. The terminal atom of the bridging species of Ni-A occupies a similar position as C of the exogenous CO in the CO complex (inhibited state). The common features of the enzyme structures at the unready (Ni-A) and inhibited (CO complex) states are proposed. These findings provide useful information on the design of new systems of biomimetic dihydrogen production and fuel cell devices.
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'''Three-Dimensional Structure Of The Ni-B State Of [Nife]Hydrogenase From Desulufovibrio Vulgaris Miyazaki F'''
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Activation process of [NiFe] hydrogenase elucidated by high-resolution X-ray analyses: conversion of the ready to the unready state.,Ogata H, Hirota S, Nakahara A, Komori H, Shibata N, Kato T, Kano K, Higuchi Y Structure. 2005 Nov;13(11):1635-42. PMID:16271886<ref>PMID:16271886</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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1WUJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WUJ OCA].
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<div class="pdbe-citations 1wuj" style="background-color:#fffaf0;"></div>
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[[Category: Cytochrome-c3 hydrogenase]]
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== References ==
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[[Category: Desulfovibrio vulgaris]]
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<references/>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Higuchi, Y.]]
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</StructureSection>
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[[Category: Hirota, S.]]
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[[Category: Desulfovibrio vulgaris str. 'Miyazaki F']]
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[[Category: Kano, K.]]
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[[Category: Large Structures]]
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[[Category: Kato, T.]]
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[[Category: Higuchi Y]]
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[[Category: Komori, H.]]
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[[Category: Hirota S]]
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[[Category: Nakahara, A.]]
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[[Category: Kano K]]
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[[Category: Ogata, H.]]
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[[Category: Kato T]]
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[[Category: Shibata, N.]]
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[[Category: Komori H]]
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[[Category: High resolution crystal structure]]
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[[Category: Nakahara A]]
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[[Category: Ni-b state]]
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[[Category: Ogata H]]
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[[Category: Oxidoreductase]]
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[[Category: Shibata N]]
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[[Category: Ready state]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 15:04:07 2008''
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Current revision

Three-Dimensional Structure Of The Ni-B State Of [Nife]Hydrogenase From Desulufovibrio Vulgaris Miyazaki F

PDB ID 1wuj

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