2cj8

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[[Image:2cj8.gif|left|200px]]<br /><applet load="2cj8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cj8, resolution 2.38&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A CELL WALL INVERTASE INHIBITOR FROM TOBACCO (PH 9.5)'''<br />
 
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==Overview==
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==Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco (pH 9.5)==
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Plant acid invertases catalyse the breakdown of sucrose. Their activity is, tightly regulated through interaction with specific protein inhibitors., The complex between the cell-wall invertase inhibitor Nt-CIF and its, target enzyme is stable only at acidic pH, as found in the plant cell, wall. Since the pH in this compartment can be modulated between pH 4 and 6, in planta, the rapid dissociation of the inhibitor-enzyme complex at, neutral pH may represent a regulatory event. Here, it is analyzed whether, the inhibitory component undergoes structural rearrangements upon changes, in the pH environment. Six crystal forms grown at pH 4.6-9.5 and, diffracting up to 1.63 Angstrom indicate only small structural changes in, CIF. This suggests that complex dissociation at neutral pH is mediated, either by rearrangements in the enzyme or by a complex pattern of surface, charges in the inhibitor-enzyme binding interface.
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<StructureSection load='2cj8' size='340' side='right'caption='[[2cj8]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2cj8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CJ8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cj8 OCA], [https://pdbe.org/2cj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cj8 RCSB], [https://www.ebi.ac.uk/pdbsum/2cj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cj8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O49908_TOBAC O49908_TOBAC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cj/2cj8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cj8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Plant acid invertases catalyse the breakdown of sucrose. Their activity is tightly regulated through interaction with specific protein inhibitors. The complex between the cell-wall invertase inhibitor Nt-CIF and its target enzyme is stable only at acidic pH, as found in the plant cell wall. Since the pH in this compartment can be modulated between pH 4 and 6 in planta, the rapid dissociation of the inhibitor-enzyme complex at neutral pH may represent a regulatory event. Here, it is analyzed whether the inhibitory component undergoes structural rearrangements upon changes in the pH environment. Six crystal forms grown at pH 4.6-9.5 and diffracting up to 1.63 Angstrom indicate only small structural changes in CIF. This suggests that complex dissociation at neutral pH is mediated either by rearrangements in the enzyme or by a complex pattern of surface charges in the inhibitor-enzyme binding interface.
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==About this Structure==
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Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range.,Hothorn M, Scheffzek K Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):665-70. Epub 2006, May 12. PMID:16699194<ref>PMID:16699194</ref>
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2CJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum] with IOD as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Iod Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CJ8 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range., Hothorn M, Scheffzek K, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):665-70. Epub 2006, May 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16699194 16699194]
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</div>
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<div class="pdbe-citations 2cj8" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Nicotiana tabacum]]
[[Category: Nicotiana tabacum]]
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[[Category: Single protein]]
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[[Category: Hothorn M]]
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[[Category: Hothorn, M.]]
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[[Category: Scheffzek K]]
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[[Category: Scheffzek, K.]]
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[[Category: IOD]]
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[[Category: four-helix bundle]]
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[[Category: helical hairpin]]
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[[Category: inhibitor]]
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[[Category: protein binding]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:25:00 2007''
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Current revision

Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco (pH 9.5)

PDB ID 2cj8

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