2cjy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2cjy" [edit=sysop:move=sysop])
Current revision (00:50, 21 November 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2cjy.png|left|200px]]
 
-
{{STRUCTURE_2cjy| PDB=2cjy | SCENE= }}
+
==Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis==
 +
<StructureSection load='2cjy' size='340' side='right'caption='[[2cjy]], [[Resolution|resolution]] 1.67&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2cjy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CJY FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0QE:CHLOROMETHANE'>0QE</scene>, <scene name='pdbligand=PHQ:BENZYL+CHLOROCARBONATE'>PHQ</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cjy OCA], [https://pdbe.org/2cjy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cjy RCSB], [https://www.ebi.ac.uk/pdbsum/2cjy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cjy ProSAT]</span></td></tr>
 +
</table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cj/2cjy_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cjy ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Caspases are cysteine proteases involved in the signalling cascades of programmed cell death in which caspase-3 plays a central role, since it propagates death signals from intrinsic and extrinsic stimuli to downstream targets. The atomic resolution (1.06 Angstroms) crystal structure of the caspase-3 DEVD-cmk complex reveals the structural basis for substrate selectivity in the S4 pocket. A low-barrier hydrogen bond is observed between the side-chains of the P4 inhibitor aspartic acid and Asp179 of the N-terminal tail of the symmetry related p12 subunit. Site-directed mutagenesis of Asp179 confirmed the significance of this residue in substrate recognition. In the 1.06 Angstroms crystal structure, a radiation damage induced rearrangement of the inhibitor methylketone moiety was observed. The carbon atom that in a substrate would represent the scissile peptide bond carbonyl carbon clearly shows a tetrahedral coordination and resembles the postulated tetrahedral intermediate of the acylation reaction.
-
===Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis===
+
Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis.,Ganesan R, Mittl PR, Jelakovic S, Grutter MG J Mol Biol. 2006 Jun 23;359(5):1378-88. Epub 2006 May 11. PMID:16787777<ref>PMID:16787777</ref>
-
{{ABSTRACT_PUBMED_16787777}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2cjy" style="background-color:#fffaf0;"></div>
-
[[2cjy]] is a 3 chain structure of [[Caspase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJY OCA].
+
==See Also==
==See Also==
-
*[[Caspase|Caspase]]
+
*[[Caspase 3D structures|Caspase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:016787777</ref><references group="xtra"/>
+
__TOC__
-
[[Category: Caspase-3]]
+
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Ganesan, R.]]
+
[[Category: Large Structures]]
-
[[Category: Grutter, M G.]]
+
[[Category: Synthetic construct]]
-
[[Category: Jelakovic, S.]]
+
[[Category: Ganesan R]]
-
[[Category: Mittl, P R.E.]]
+
[[Category: Grutter MG]]
-
[[Category: Apoptosis]]
+
[[Category: Jelakovic S]]
-
[[Category: Clan cd]]
+
[[Category: Mittl PRE]]
-
[[Category: Cysteine protease]]
+
-
[[Category: Hydrolase-hydrolase inhibitor complex]]
+
-
[[Category: Ice]]
+
-
[[Category: Protease]]
+
-
[[Category: Safety catch]]
+
-
[[Category: Thiol protease]]
+

Current revision

Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis

PDB ID 2cjy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cjy"
Personal tools