2hhi
From Proteopedia
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| - | [[Image:2hhi.gif|left|200px]] | ||
| - | + | ==The solution structure of antigen MPT64 from Mycobacterium tuberculosis defines a novel class of beta-grasp proteins== | |
| - | + | <StructureSection load='2hhi' size='340' side='right'caption='[[2hhi]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2hhi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HHI FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 24 models</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hhi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hhi OCA], [https://pdbe.org/2hhi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hhi RCSB], [https://www.ebi.ac.uk/pdbsum/2hhi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hhi ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | == Function == | |
| - | + | [https://www.uniprot.org/uniprot/MP64_MYCTU MP64_MYCTU] | |
| - | + | == Evolutionary Conservation == | |
| - | + | [[Image:Consurf_key_small.gif|200px|right]] | |
| - | == | + | Check<jmol> |
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hhi_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hhi ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
The MPT64 protein and its homologs form a highly conserved family of secreted proteins with unknown function that are found within the pathogenic Mycobacteria genus. The founding member of this family from Mycobacterium tuberculosis (MPT64 or protein Rv1980c) is expressed only when Mycobacteria cells are actively dividing. By virtue of this relatively unique expression profile, Rv1980c is currently under phase III clinical trials to evaluate its potential to replace tuberculin, or purified protein derivative, as the rapid diagnostic of choice for detection of active tuberculosis infection. We describe here the NMR solution structure of Rv1980c. This structure reveals a previously undescribed fold that is based upon a variation of a beta-grasp motif most commonly found in protein-protein interaction domains. Examination of this structure in conjunction with multiple sequence alignments of MPT64 homologs identifies a candidate ligand-binding site, which may help guide future studies of Rv1980c function. The work presented here also suggests structure-based approaches for increasing the antigenic potency of a Rv1980c-based diagnostic. | The MPT64 protein and its homologs form a highly conserved family of secreted proteins with unknown function that are found within the pathogenic Mycobacteria genus. The founding member of this family from Mycobacterium tuberculosis (MPT64 or protein Rv1980c) is expressed only when Mycobacteria cells are actively dividing. By virtue of this relatively unique expression profile, Rv1980c is currently under phase III clinical trials to evaluate its potential to replace tuberculin, or purified protein derivative, as the rapid diagnostic of choice for detection of active tuberculosis infection. We describe here the NMR solution structure of Rv1980c. This structure reveals a previously undescribed fold that is based upon a variation of a beta-grasp motif most commonly found in protein-protein interaction domains. Examination of this structure in conjunction with multiple sequence alignments of MPT64 homologs identifies a candidate ligand-binding site, which may help guide future studies of Rv1980c function. The work presented here also suggests structure-based approaches for increasing the antigenic potency of a Rv1980c-based diagnostic. | ||
| - | + | The solution structure of antigen MPT64 from Mycobacterium tuberculosis defines a new family of beta-grasp proteins.,Wang Z, Potter BM, Gray AM, Sacksteder KA, Geisbrecht BV, Laity JH J Mol Biol. 2007 Feb 16;366(2):375-81. Epub 2006 Nov 14. PMID:17174329<ref>PMID:17174329</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 2hhi" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Geisbrecht | + | <references/> |
| - | [[Category: Gray | + | __TOC__ |
| - | [[Category: Laity | + | </StructureSection> |
| - | [[Category: Potter | + | [[Category: Large Structures]] |
| - | [[Category: Sacksteder | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Wang | + | [[Category: Geisbrecht BV]] |
| - | + | [[Category: Gray AM]] | |
| - | + | [[Category: Laity JH]] | |
| - | + | [[Category: Potter BM]] | |
| - | + | [[Category: Sacksteder KA]] | |
| - | + | [[Category: Wang Z]] | |
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Current revision
The solution structure of antigen MPT64 from Mycobacterium tuberculosis defines a novel class of beta-grasp proteins
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