2k38

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{{Seed}}
 
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[[Image:2k38.png|left|200px]]
 
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==Cupiennin 1A, NMR, minimized average structure==
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The line below this paragraph, containing "STRUCTURE_2k38", creates the "Structure Box" on the page.
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<StructureSection load='2k38' size='340' side='right'caption='[[2k38]]' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2k38]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupiennius_salei Cupiennius salei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K38 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 1 model</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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{{STRUCTURE_2k38| PDB=2k38 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k38 OCA], [https://pdbe.org/2k38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k38 RCSB], [https://www.ebi.ac.uk/pdbsum/2k38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k38 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TXC1A_CUPSA TXC1A_CUPSA] Has antimicrobial activity against B.subtilis, E.coli, E.faecalis, P.denitrificans, P.aeruginosa, P.putida, S.aureus, and S.epidermidis. Has insecticidal and hemolytic activities. Probably acts by disturbing membrane function with its amphipathic structure. Synergistically increases the insecticidal activity of CSTX-1, CSTX-9, and CSTX-13 by up to 65%. Also inhibits the formation of nitric oxide by neuronal nitric oxide synthase.<ref>PMID:11792701</ref> <ref>PMID:10669026</ref> <ref>PMID:15914655</ref> <ref>PMID:17313650</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The solution structure of cupiennin 1a, a 35 residue, basic antibacterial peptide isolated from the venom of the spider Cupiennius salei, has been determined by nuclear magnetic resonance (NMR) spectroscopy. The peptide was found to adopt a helix-hinge-helix structure in a membrane mimicking solvent. The hinge may play a role in allowing the amphipathic N-terminal helix and polar C-terminal helix to orient independently upon membrane binding, in order to achieve maximal antibacterial efficacy. Solid-state 31P and 2H NMR was used to further study the effects of cupiennin 1a on the dynamic properties of lipid membranes, using zwitterionic chain deuterated dimyristoylphosphatidylcholine (d54-DMPC) and anionic dimyristoylphosphatidylglycerol (DMPG) multilamellar vesicles. In d54-DMPC alone, cupiennin 1a caused a decrease in the 31P chemical shift anisotropy, indicating some interaction with the lipid head groups, and a decrease in order over the entire acyl chain. In contrast, for the mixed (d54-DMPC/DMPG) lipid system cupiennin 1a appeared to induce lateral separation of the two lipids as evidenced by the 31P spectra, in which the peptide preferentially interacted with DMPG. Little effect was observed on the deuterated acyl chain order parameters in the d54-DMPC/DMPG model membranes. Furthermore, 31P NMR relaxation measurements confirmed a differential effect on the lipid motions depending upon the membrane composition. Therefore, subtle differences are likely in the mechanism by which cupiennin 1a causes membrane lysis in either prokaryotic or eukaryotic cells, and may explain the specific spectrum of activity.
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===Cupiennin 1A, NMR, minimized average structure===
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Solution structure and interaction of cupiennin 1a, a spider venom peptide, with phospholipid bilayers.,Pukala TL, Boland MP, Gehman JD, Kuhn-Nentwig L, Separovic F, Bowie JH Biochemistry. 2007 Mar 20;46(11):3576-85. Epub 2007 Feb 24. PMID:17319697<ref>PMID:17319697</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_17319697}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2k38" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17319697 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17319697}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2K38 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cupiennius_salei Cupiennius salei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K38 OCA].
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==Reference==
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Solution structure and interaction of cupiennin 1a, a spider venom peptide, with phospholipid bilayers., Pukala TL, Boland MP, Gehman JD, Kuhn-Nentwig L, Separovic F, Bowie JH, Biochemistry. 2007 Mar 20;46(11):3576-85. Epub 2007 Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17319697 17319697]
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[[Category: Cupiennius salei]]
[[Category: Cupiennius salei]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Boland, M P.]]
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[[Category: Boland MP]]
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[[Category: Bowie, J H.]]
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[[Category: Bowie JH]]
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[[Category: Gehman, J D.]]
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[[Category: Gehman JD]]
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[[Category: Kuhn-Nentwig, L.]]
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[[Category: Kuhn-Nentwig L]]
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[[Category: Pukala, T L.]]
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[[Category: Pukala TL]]
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[[Category: Separovic, F.]]
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[[Category: Separovic F]]
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[[Category: Amidation]]
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[[Category: Antibacterial]]
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[[Category: Antibiotic]]
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[[Category: Antimicrobial]]
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[[Category: Antimicrobial protein]]
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[[Category: Cytolysis]]
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[[Category: Helix-hinge-helix]]
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[[Category: Hemolysis]]
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[[Category: Membrane active]]
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[[Category: Neurotoxin]]
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[[Category: Secreted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:06:50 2008''
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Current revision

Cupiennin 1A, NMR, minimized average structure

PDB ID 2k38

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