3ezh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (01:47, 21 November 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3ezh.png|left|200px]]
 
-
{{STRUCTURE_3ezh| PDB=3ezh | SCENE= }}
+
==Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain in Complex with Nitrate==
 +
<StructureSection load='3ezh' size='340' side='right'caption='[[3ezh]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3ezh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EZH FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ezh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ezh OCA], [https://pdbe.org/3ezh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ezh RCSB], [https://www.ebi.ac.uk/pdbsum/3ezh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ezh ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/NARX_ECOLI NARX_ECOLI] Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the NarL protein and of both nitrate/nitrite to the NarP protein. NarX probably activates NarL and NarP by phosphorylation in the presence of nitrate. NarX also plays a negative role in controlling NarL activity, probably through dephosphorylation in the absence of nitrate.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/3ezh_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ezh ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Histidine kinase receptors are a large family of membrane-spanning proteins found in many prokaryotes and some eukaryotes. They are a part of two-component signal transduction systems, which each comprise a sensor kinase and a response regulator and are involved with the regulation of many cellular processes. NarX is a histidine kinase receptor that responds to nitrate and nitrite to effect regulation of anaerobic respiration in various bacteria. We present high-resolution X-ray crystal structures of the periplasmic sensor domain from Escherichia coli NarX in a complex with nitrate and in the apo state. Our analysis reveals that nitrate-binding induces conformation changes that result in a piston-type displacement between the N- and C-terminal helices of the periplasmic domain. Such conformational changes might represent a conserved mechanism of signaling in histidine kinases by which ligand binding is communicated across the lipid bilayer.
-
===Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain in Complex with Nitrate===
+
Structural Analysis of Ligand Stimulation of the Histidine Kinase NarX.,Cheung J, Hendrickson WA Structure. 2009 Feb 13;17(2):190-201. PMID:19217390<ref>PMID:19217390</ref>
-
{{ABSTRACT_PUBMED_19217390}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 3ezh" style="background-color:#fffaf0;"></div>
-
[[3ezh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EZH OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:019217390</ref><references group="xtra"/>
+
</StructureSection>
-
[[Category: Escherichia coli k-12]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Histidine kinase]]
+
[[Category: Large Structures]]
-
[[Category: Cheung, J.]]
+
[[Category: Cheung J]]
-
[[Category: Hendrickson, W A.]]
+
[[Category: Hendrickson WA]]
-
[[Category: Cell inner membrane]]
+
-
[[Category: Cell membrane]]
+
-
[[Category: Four-helix bundle]]
+
-
[[Category: Histidine kinase]]
+
-
[[Category: Kinase]]
+
-
[[Category: Membrane]]
+
-
[[Category: Nitrate assimilation]]
+
-
[[Category: Nitrate sensor]]
+
-
[[Category: Phosphoprotein]]
+
-
[[Category: Selenomethionyl mad]]
+
-
[[Category: Sensor domain]]
+
-
[[Category: Sensor protein]]
+
-
[[Category: Signal transduction]]
+
-
[[Category: Transferase]]
+
-
[[Category: Transmembrane]]
+
-
[[Category: Two-component regulatory system]]
+

Current revision

Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain in Complex with Nitrate

PDB ID 3ezh

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ezh"
Personal tools