3ezh

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[[Image:3ezh.png|left|200px]]
 
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{{STRUCTURE_3ezh| PDB=3ezh | SCENE= }}
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==Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain in Complex with Nitrate==
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<StructureSection load='3ezh' size='340' side='right'caption='[[3ezh]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ezh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EZH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ezh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ezh OCA], [https://pdbe.org/3ezh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ezh RCSB], [https://www.ebi.ac.uk/pdbsum/3ezh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ezh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NARX_ECOLI NARX_ECOLI] Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the NarL protein and of both nitrate/nitrite to the NarP protein. NarX probably activates NarL and NarP by phosphorylation in the presence of nitrate. NarX also plays a negative role in controlling NarL activity, probably through dephosphorylation in the absence of nitrate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/3ezh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ezh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Histidine kinase receptors are a large family of membrane-spanning proteins found in many prokaryotes and some eukaryotes. They are a part of two-component signal transduction systems, which each comprise a sensor kinase and a response regulator and are involved with the regulation of many cellular processes. NarX is a histidine kinase receptor that responds to nitrate and nitrite to effect regulation of anaerobic respiration in various bacteria. We present high-resolution X-ray crystal structures of the periplasmic sensor domain from Escherichia coli NarX in a complex with nitrate and in the apo state. Our analysis reveals that nitrate-binding induces conformation changes that result in a piston-type displacement between the N- and C-terminal helices of the periplasmic domain. Such conformational changes might represent a conserved mechanism of signaling in histidine kinases by which ligand binding is communicated across the lipid bilayer.
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===Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain in Complex with Nitrate===
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Structural Analysis of Ligand Stimulation of the Histidine Kinase NarX.,Cheung J, Hendrickson WA Structure. 2009 Feb 13;17(2):190-201. PMID:19217390<ref>PMID:19217390</ref>
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{{ABSTRACT_PUBMED_19217390}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3ezh" style="background-color:#fffaf0;"></div>
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[[3ezh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EZH OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019217390</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Escherichia coli k-12]]
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[[Category: Escherichia coli K-12]]
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[[Category: Histidine kinase]]
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[[Category: Large Structures]]
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[[Category: Cheung, J.]]
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[[Category: Cheung J]]
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[[Category: Hendrickson, W A.]]
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[[Category: Hendrickson WA]]
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[[Category: Cell inner membrane]]
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[[Category: Cell membrane]]
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[[Category: Four-helix bundle]]
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[[Category: Histidine kinase]]
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[[Category: Kinase]]
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[[Category: Membrane]]
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[[Category: Nitrate assimilation]]
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[[Category: Nitrate sensor]]
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[[Category: Phosphoprotein]]
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[[Category: Selenomethionyl mad]]
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[[Category: Sensor domain]]
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[[Category: Sensor protein]]
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[[Category: Signal transduction]]
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[[Category: Transferase]]
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[[Category: Transmembrane]]
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[[Category: Two-component regulatory system]]
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Current revision

Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain in Complex with Nitrate

PDB ID 3ezh

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