3lob

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[[Image:3lob.png|left|200px]]
 
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==Crystal Structure of Flock House Virus calcium mutant==
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The line below this paragraph, containing "STRUCTURE_3lob", creates the "Structure Box" on the page.
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<StructureSection load='3lob' size='340' side='right'caption='[[3lob]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3lob]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Flock_House_virus Flock House virus] and [https://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LOB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P:2-DEOXY-N1,N2-PROPANO+GUANOSINE+MONOPHOSPHATE'>P</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3lob| PDB=3lob | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lob OCA], [https://pdbe.org/3lob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lob RCSB], [https://www.ebi.ac.uk/pdbsum/3lob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lob ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSD_FHV CAPSD_FHV] Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. In addition, 240 calcium ions are incorporated per capsid during assembly. The capsid encapsulates the two genomic RNAs. Capsid maturation occurs via autoproteolytic cleavage of capsid protein alpha generating capsid protein beta and the membrane-active peptide gamma. Peptide gamma: membrane-permeabilizing peptide produced by virus maturation, thereby creating the infectious virion. After endocytosis into the host cell, peptide gamma is probably exposed in endosomes, where it permeabilizes the endosomal membrane, facilitating translocation of viral capsid or RNA into the cytoplasm (Probable).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lo/3lob_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lob ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Divalent metal ions are components of numerous icosahedral virus capsids. Flock House virus (FHV), a small RNA virus of the family Nodaviridae, was utilized as an accessible model system with which to address the effects of metal ions on capsid structure and on the biology of virus-host interactions. Mutations at the calcium-binding sites affected FHV capsid stability and drastically reduced virus infectivity, without altering the overall architecture of the capsid. The mutations also altered the conformation of gamma, a membrane-disrupting, virus-encoded peptide usually sequestered inside the capsid, by increasing its exposure under neutral pH conditions. Our data demonstrate that calcium binding is essential for maintaining a pH-based control on gamma exposure and host membrane disruption, and they reveal a novel rationale for the metal ion requirement during virus entry and infectivity. In the light of the phenotypes displayed by a calcium site mutant of FHV, we suggest that this mutant corresponds to an early entry intermediate formed in the endosomal pathway.
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===Crystal Structure of Flock House Virus calcium mutant===
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Structure and function of a genetically engineered mimic of a nonenveloped virus entry intermediate.,Banerjee M, Speir JA, Kwan MH, Huang R, Aryanpur PP, Bothner B, Johnson JE J Virol. 2010 May;84(9):4737-46. Epub 2010 Feb 17. PMID:20164221<ref>PMID:20164221</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3lob" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20164221}}, adds the Publication Abstract to the page
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*[[Flock house virus protein B2|Flock house virus protein B2]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20164221 is the PubMed ID number.
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_20164221}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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[[3lob]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Flock_house_virus Flock house virus] and [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOB OCA].
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[[Category: Flock House virus]]
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:20164221</ref><references group="xtra"/>
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[[Category: Flock house virus]]
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[[Category: Nodavirus endopeptidase]]
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[[Category: Spodoptera frugiperda]]
[[Category: Spodoptera frugiperda]]
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[[Category: Banerjee, M.]]
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[[Category: Banerjee M]]
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[[Category: Huang, R.]]
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[[Category: Huang R]]
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[[Category: Johnson, J E.]]
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[[Category: Johnson JE]]
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[[Category: Speir, J A.]]
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[[Category: Speir JA]]
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[[Category: Aspartyl protease]]
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[[Category: Beta-barrel]]
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[[Category: Capsid protein]]
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[[Category: Flock house virus]]
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[[Category: Hydrolase]]
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[[Category: Icosahedral virus]]
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[[Category: Jellyroll]]
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[[Category: Protease]]
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[[Category: Rna]]
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[[Category: Virion]]
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[[Category: Virus]]
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Current revision

Crystal Structure of Flock House Virus calcium mutant

PDB ID 3lob

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Proteopedia Page Contributors and Editors (what is this?)

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