3w3e
From Proteopedia
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==Structure of Vigna unguiculata chitinase with regulation activity of the plant cell wall== | ==Structure of Vigna unguiculata chitinase with regulation activity of the plant cell wall== | ||
| - | <StructureSection load='3w3e' size='340' side='right' caption='[[3w3e]], [[Resolution|resolution]] 1.50Å' scene=''> | + | <StructureSection load='3w3e' size='340' side='right'caption='[[3w3e]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3w3e]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3w3e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vigna_unguiculata Vigna unguiculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W3E FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w3e OCA], [https://pdbe.org/3w3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w3e RCSB], [https://www.ebi.ac.uk/pdbsum/3w3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w3e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8H0C9_VIGUN Q8H0C9_VIGUN] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
We examined the distribution and the immunohistochemical localization of yieldin in etiolated cowpea seedlings with an anti-yieldin antibody. An immunoblotting analysis revealed that the yieldin was located in the aerial organs (plumule, epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin signal in the hypocotyls was highest in the apical pre-elongation region (the hook region) and decreased toward the elongated mature base indicating that the yieldin disappeared with the ceasing of cell elongation. Tissue-print immunoblotting analysis using hypocotyls in different germination stages supports this view because the apical yieldin-rich regions, just beneath the cotyledonary node (the hook and rapidly elongating regions), acropetally migrated together with hypocotyl elongation. Immunohistochemical microscopy demonstrated that yieldin was localized in the cell walls of the cortex and epidermis of the germ axes. The present results are consistent with the view that yieldin participates in the regulation of cell wall yielding during elongation growth. | We examined the distribution and the immunohistochemical localization of yieldin in etiolated cowpea seedlings with an anti-yieldin antibody. An immunoblotting analysis revealed that the yieldin was located in the aerial organs (plumule, epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin signal in the hypocotyls was highest in the apical pre-elongation region (the hook region) and decreased toward the elongated mature base indicating that the yieldin disappeared with the ceasing of cell elongation. Tissue-print immunoblotting analysis using hypocotyls in different germination stages supports this view because the apical yieldin-rich regions, just beneath the cotyledonary node (the hook and rapidly elongating regions), acropetally migrated together with hypocotyl elongation. Immunohistochemical microscopy demonstrated that yieldin was localized in the cell walls of the cortex and epidermis of the germ axes. The present results are consistent with the view that yieldin participates in the regulation of cell wall yielding during elongation growth. | ||
| - | Distribution of yieldin, a regulatory protein of the cell wall yield threshold, in etiolated cowpea seedlings.,Okamoto-Nakazato A, Takahashi K, Katoh-Semba R, Katou K Plant Cell Physiol. 2001 Sep;42(9):952-8. PMID: | + | Distribution of yieldin, a regulatory protein of the cell wall yield threshold, in etiolated cowpea seedlings.,Okamoto-Nakazato A, Takahashi K, Katoh-Semba R, Katou K Plant Cell Physiol. 2001 Sep;42(9):952-8. PMID:011577189<ref>PMID:011577189</ref> |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
| + | <div class="pdbe-citations 3w3e" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Chitinase|Chitinase]] | + | *[[Chitinase 3D structures|Chitinase 3D structures]] |
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Vigna unguiculata]] | [[Category: Vigna unguiculata]] | ||
| - | [[Category: Morohashi | + | [[Category: Morohashi K]] |
| - | [[Category: Sakabe | + | [[Category: Sakabe K]] |
| - | [[Category: Sakabe | + | [[Category: Sakabe N]] |
| - | [[Category: Sasaki | + | [[Category: Sasaki K]] |
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Current revision
Structure of Vigna unguiculata chitinase with regulation activity of the plant cell wall
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