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| | ==Staphyloferrin B precursor biosynthetic enzyme selenomethionine-labeled SbnB== | | ==Staphyloferrin B precursor biosynthetic enzyme selenomethionine-labeled SbnB== |
| - | <StructureSection load='4mp3' size='340' side='right' caption='[[4mp3]], [[Resolution|resolution]] 2.11Å' scene=''> | + | <StructureSection load='4mp3' size='340' side='right'caption='[[4mp3]], [[Resolution|resolution]] 2.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mp3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staaw Staaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MP3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MP3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mp3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_MW2 Staphylococcus aureus subsp. aureus MW2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MP3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MP3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m54|4m54]], [[4mp6|4mp6]], [[4mp8|4mp8]], [[4mpd|4mpd]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mp3 OCA], [https://pdbe.org/4mp3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mp3 RCSB], [https://www.ebi.ac.uk/pdbsum/4mp3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mp3 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sbnB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 STAAW])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mp3 OCA], [http://pdbe.org/4mp3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mp3 RCSB], [http://www.ebi.ac.uk/pdbsum/4mp3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mp3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Staaw]] | + | [[Category: Large Structures]] |
| - | [[Category: Grigg, J C]] | + | [[Category: Staphylococcus aureus subsp. aureus MW2]] |
| - | [[Category: Kobylarz, M J]]
| + | [[Category: Grigg JC]] |
| - | [[Category: Murphy, M E.P]]
| + | [[Category: Kobylarz MJ]] |
| - | [[Category: Rai, D K]] | + | [[Category: Murphy MEP]] |
| - | [[Category: Acega dehydrogenase]] | + | [[Category: Rai DK]] |
| - | [[Category: L-dap synthesis]] | + | |
| - | [[Category: Oxidoreductase]] | + | |
| - | [[Category: Siderophore]]
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| Structural highlights
Publication Abstract from PubMed
L-2,3-diaminopropionic acid (L-Dap) is an amino acid that is a precursor of antibiotics and staphyloferrin B a siderophore produced by Staphylococcus aureus. SbnA and SbnB are encoded by the staphyloferrin B biosynthetic gene cluster and are implicated in L-Dap biosynthesis. We demonstrate here that SbnA uses PLP and substrates O-phospho-L-serine and L-glutamate to produce a metabolite N-(1-amino-1-carboxyl-2-ethyl)-glutamic acid (ACEGA). SbnB is shown to use NAD(+) to oxidatively hydrolyze ACEGA to yield alpha-ketoglutarate and L-Dap. Also, we describe crystal structures of SbnB in complex with NADH and ACEGA as well as with NAD(+) and alpha-ketoglutarate to reveal the residues required for substrate binding, oxidation, and hydrolysis. SbnA and SbnB contribute to the iron sparing response of S. aureus that enables staphyloferrin B biosynthesis in the absence of an active tricarboxylic acid cycle.
Synthesis of L-2,3-diaminopropionic Acid, a siderophore and antibiotic precursor.,Kobylarz MJ, Grigg JC, Takayama SJ, Rai DK, Heinrichs DE, Murphy ME Chem Biol. 2014 Mar 20;21(3):379-88. doi: 10.1016/j.chembiol.2013.12.011. Epub, 2014 Jan 30. PMID:24485762[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kobylarz MJ, Grigg JC, Takayama SJ, Rai DK, Heinrichs DE, Murphy ME. Synthesis of L-2,3-diaminopropionic Acid, a siderophore and antibiotic precursor. Chem Biol. 2014 Mar 20;21(3):379-88. doi: 10.1016/j.chembiol.2013.12.011. Epub, 2014 Jan 30. PMID:24485762 doi:http://dx.doi.org/10.1016/j.chembiol.2013.12.011
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