4us5
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of apo-MsnO8== | |
| + | <StructureSection load='4us5' size='340' side='right'caption='[[4us5]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4us5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_bottropensis Streptomyces bottropensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4US5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4US5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P4G:1-ETHOXY-2-(2-ETHOXYETHOXY)ETHANE'>P4G</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4us5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4us5 OCA], [https://pdbe.org/4us5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4us5 RCSB], [https://www.ebi.ac.uk/pdbsum/4us5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4us5 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/W8QCH3_9ACTN W8QCH3_9ACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mensacarcin, a potential antitumour drug, is produced by Streptomyces bottropensis. The structure consists of a three-membered ring system with many oxygen atoms. Of vital importance in this context is an epoxy moiety in the side chain of mensacarcin. Our studies with different mensacarcin derivatives have demonstrated that this epoxy group is primarily responsible for the cytotoxic effect of mensacarcin. In order to obtain further information about this epoxy moiety, inactivation experiments in the gene cluster were carried out to identify the epoxy-forming enzyme. Therefore the cosmid cos2, which covers almost the complete type II polyketide synthase (PKS) gene cluster, was heterologously expressed in Streptomyces albus. This led to production of didesmethylmensacarcin, due to the fact that methyltransferase genes are missing in the cosmid. Further gene inactivation experiments on this cosmid showed that MsnO8, a luciferase-like monooxygenase, introduces the epoxy group at the end of the biosynthesis of mensacarcin. In addition, the protein MsnO8 was purified, and its crystal structure was determined to a resolution of 1.80 A. | ||
| - | + | Insights into the bioactivity of mensacarcin and epoxide formation by MsnO8.,Maier S, Pfluger T, Loesgen S, Asmus K, Brotz E, Paululat T, Zeeck A, Andrade S, Bechthold A Chembiochem. 2014 Mar 21;15(5):749-56. doi: 10.1002/cbic.201300704. Epub 2014 Feb, 19. PMID:24554499<ref>PMID:24554499</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4us5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces bottropensis]] | ||
| + | [[Category: Andrade S]] | ||
| + | [[Category: Asmus K]] | ||
| + | [[Category: Bechthold A]] | ||
| + | [[Category: Broetz E]] | ||
| + | [[Category: Loesgen S]] | ||
| + | [[Category: Maier S]] | ||
| + | [[Category: Paululat T]] | ||
| + | [[Category: Pflueger T]] | ||
| + | [[Category: Zeeck A]] | ||
Current revision
Crystal Structure of apo-MsnO8
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