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1ukm

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[[Image:1ukm.gif|left|200px]]
[[Image:1ukm.gif|left|200px]]
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{{Structure
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|PDB= 1ukm |SIZE=350|CAPTION= <scene name='initialview01'>1ukm</scene>, resolution 1.90&Aring;
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ukm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ukm OCA], [http://www.ebi.ac.uk/pdbsum/1ukm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ukm RCSB]</span>
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'''Crystal structure of EMS16, an Antagonist of collagen receptor integrin alpha2beta1 (GPIa/IIa)'''
'''Crystal structure of EMS16, an Antagonist of collagen receptor integrin alpha2beta1 (GPIa/IIa)'''
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[[Category: Morita, T.]]
[[Category: Morita, T.]]
[[Category: Okuda, D.]]
[[Category: Okuda, D.]]
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[[Category: c-type lectin]]
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[[Category: C-type lectin]]
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[[Category: domain swapping]]
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[[Category: Domain swapping]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:21:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:11:33 2008''
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Revision as of 08:21, 3 May 2008

Template:STRUCTURE 1ukm

Crystal structure of EMS16, an Antagonist of collagen receptor integrin alpha2beta1 (GPIa/IIa)


Overview

Snake venoms contain a number of hemostatically active C-type lectin-like proteins (CLPs), which affect the blood coagulation system, endothelial cells, and platelets. CLPs have broad similarities in structure and possess distinct biological functions. EMS16, a CLP from Echis multisquamatus venom, which is a potent and selective inhibitor of the collagen receptor, glycoprotein Ia/IIa (integrin alpha2beta1), has been used in the present study to examine structure-function relationships in venom CLPs by X-ray crystallography. The structure of EMS16, determined at a resolution of 1.9 A, revealed a heterodimer involved with domain swapping of the central loop as observed in the structures of other CLPs. A part of the glycan was observed and identified as N-acetyl-D-glucosamine (GlcNAc) in the electron density map at Asn21 of subunit B, an expected glycosylation site. EMS16 had a unique, positively charged electrostatic potential patch on the concave surface that may qualify as a site for interaction with the I-domain of the glycoprotein Ia/IIa.

About this Structure

1UKM is a Protein complex structure of sequences from Echis multisquamatus. Full crystallographic information is available from OCA.

Reference

Structural characterization of EMS16, an antagonist of collagen receptor (GPIa/IIa) from the venom of Echis multisquamatus., Horii K, Okuda D, Morita T, Mizuno H, Biochemistry. 2003 Nov 4;42(43):12497-502. PMID:14580195 Page seeded by OCA on Sat May 3 11:21:35 2008

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