BtuB

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BtuB is an outer membrane receptor found in a variety of bacteria, such as ''E. coli''. As an essential receptor for the cell that is constitutively expressed, it is an ideal target to be parasitized, a feature exploited by a variety of proteins such as [[Colicin]]s.
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<StructureSection load='3m8d' size='450' side='right' scene='43/439262/Cv/1' caption='E. coli BtuB complex with cobalamin (a vitamin B12 derivative), lipid, methanesulfonothioate derivative and Ca+2 ions (green) (PDB code [[3m8d]])'>
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{{STRUCTURE_2guf | PDB=2guf | SCENE= BtuB/Btub/1 }}
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== Function ==
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'''BtuB''' or '''vitamin B12 receptor''' or '''outer membrane cobalamin transporter''' is an outer membrane receptor found in a variety of bacteria, such as ''E. coli''. BtuB transports vitamin B12 across the membrane of gram-negative bacteria. The transport is achieved with high affinity by the collaboration of BtuB and the periplasmic protein TonB. <ref>PMID:14499604</ref> As an essential receptor for the cell that is constitutively expressed, it is an ideal target to be parasitized, a feature exploited by a variety of proteins such as [[Colicin]]s.
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== Structural highlights ==
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BtuB depends on the presence of Ca+2 ions for high affinity <scene name='43/439262/Cv/6'>binding of cobalamin (a form of vitamin B12)</scene>. The <scene name='43/439262/Cv/7'>Ca+2 ions are coordinated to several Asp side chains</scene>.<ref>PMID:20816073</ref>
==3D structure of BtuB==
==3D structure of BtuB==
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[[BtuB 3D structures]]
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[[2guf]], [[1nqe]], [[1nqf]] – EcBtuB – ''Escherichia coli''<br />
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</StructureSection>
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[[3m8b]] – EcBtuB (mutant) <br />
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[[3m8d]] - EcBtuB (mutant) + cyanocobalamin<br />
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[[1nqh]] - EcBtuB + cyanocobalamin + Ca<br />
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== References ==
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[[2ysu]] - EcBtuB + Colicin E2 receptor binding domain<br />
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<references/>
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[[1ujw]] - EcBtuB + Colicin E3 receptor binding domain<br />
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[[Category:Topic Page]]
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[[2gsk]] - EcBtuB + TonB
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Current revision

E. coli BtuB complex with cobalamin (a vitamin B12 derivative), lipid, methanesulfonothioate derivative and Ca+2 ions (green) (PDB code 3m8d)

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References

  1. ↑ Chimento DP, Kadner RJ, Wiener MC. The Escherichia coli outer membrane cobalamin transporter BtuB: structural analysis of calcium and substrate binding, and identification of orthologous transporters by sequence/structure conservation. J Mol Biol. 2003 Oct 3;332(5):999-1014. PMID:14499604 doi:http://dx.doi.org/10.1016/S0022283603009975
  2. ↑ Freed DM, Horanyi PS, Wiener MC, Cafiso DS. Conformational exchange in a membrane transport protein is altered in protein crystals. Biophys J. 2010 Sep 8;99(5):1604-10. PMID:20816073 doi:10.1016/j.bpj.2010.06.026

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Gemma McGoldrick

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