2hp4

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{{Seed}}
 
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[[Image:2hp4.png|left|200px]]
 
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==Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor==
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The line below this paragraph, containing "STRUCTURE_2hp4", creates the "Structure Box" on the page.
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<StructureSection load='2hp4' size='340' side='right'caption='[[2hp4]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2hp4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HP4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2hp4| PDB=2hp4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hp4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp4 OCA], [https://pdbe.org/2hp4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hp4 RCSB], [https://www.ebi.ac.uk/pdbsum/2hp4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hp4 ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/CD8A_HUMAN CD8A_HUMAN] Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:[https://omim.org/entry/608957 608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
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== Function ==
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[https://www.uniprot.org/uniprot/CD8A_HUMAN CD8A_HUMAN] Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/2hp4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hp4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human CD8 is a T cell coreceptor, which binds to pHLA I and plays a pivotal role in the activation of cytotoxic T lymphocytes. Soluble recombinant CD8 alphaalpha has been shown to antagonize T cell activation, both in vitro and in vivo. However, because of a very low affinity for pHLA I, high concentrations of soluble CD8 alphaalpha are required for efficient inhibition. Based upon our knowledge of the wild-type CD8/pHLA I structure, we have designed and produced a mutated form of soluble CD8 alphaalpha that binds to pHLA I with approximately fourfold higher affinity. We have characterized the binding of the high affinity CD8 mutant using surface plasmon resonance and determined its structure at 2.1 A resolution using X-ray crystallography. The analysis of this structure suggests that the higher affinity is achieved by providing a larger side chain that allows for an optimal contact to be made between the HLA alpha3 loop and the mutated CDR-like loops of CD8.
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===Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor===
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Computational design and crystal structure of an enhanced affinity mutant human CD8 alphaalpha coreceptor.,Cole DK, Rizkallah PJ, Boulter JM, Sami M, Vuidepot AL, Glick M, Gao F, Bell JI, Jakobsen BK, Gao GF Proteins. 2007 Apr 1;67(1):65-74. PMID:17243170<ref>PMID:17243170</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2hp4" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17243170}}, adds the Publication Abstract to the page
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*[[CD8 3D structures|CD8 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17243170 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17243170}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2HP4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP4 OCA].
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==Reference==
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Computational design and crystal structure of an enhanced affinity mutant human CD8 alphaalpha coreceptor., Cole DK, Rizkallah PJ, Boulter JM, Sami M, Vuidepot AL, Glick M, Gao F, Bell JI, Jakobsen BK, Gao GF, Proteins. 2007 Apr 1;67(1):65-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17243170 17243170]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Boulter, J M.]]
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[[Category: Boulter JM]]
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[[Category: Cole, D K.]]
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[[Category: Cole DK]]
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[[Category: Gao, G F.]]
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[[Category: Gao GF]]
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[[Category: Glick, M.]]
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[[Category: Glick M]]
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[[Category: Jakobsen, B K.]]
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[[Category: Jakobsen BK]]
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[[Category: Rizkallah, P J.]]
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[[Category: Rizkallah PJ]]
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[[Category: Cd8]]
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[[Category: Co-receptor]]
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[[Category: Crystal structure]]
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[[Category: Immune-suppressor]]
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[[Category: Immunotherapy]]
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[[Category: Kd]]
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[[Category: Protein engineering]]
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[[Category: Soluble protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:51:04 2008''
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Current revision

Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor

PDB ID 2hp4

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