5t5h

From Proteopedia

(Difference between revisions)

Current revision

Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit

5t5h, resolution 2.54Å

Structural highlights

5t5h is a 10 chain structure with sequence from Trypanosoma cruzi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 2.54Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL18_TRYCC

Publication Abstract from PubMed

Ribosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA expansion segments, and r-protein variations compared with other eukaryotic ribosomes. To investigate the architecture of the trypanosomatid ribosomes, we determined the 2.5-A structure of the Trypanosoma cruzi ribosome large subunit by single-particle cryo-EM. Examination of this structure and comparative analysis of the yeast ribosomal assembly pathway allowed us to develop a stepwise assembly model for the eight pieces of the large subunit rRNAs and a number of ancillary "glue" proteins. This model can be applied to the characterization of Trypanosoma brucei and Leishmania spp. ribosomes as well. Together with other details, our atomic-level structure may provide a foundation for structure-based design of antitrypanosome drugs.

Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit.,Liu Z, Gutierrez-Vargas C, Wei J, Grassucci RA, Ramesh M, Espina N, Sun M, Tutuncuoglu B, Madison-Antenucci S, Woolford JL Jr, Tong L, Frank J Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12174-12179. Epub 2016 Oct 10. PMID:27791004^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu Z, Gutierrez-Vargas C, Wei J, Grassucci RA, Ramesh M, Espina N, Sun M, Tutuncuoglu B, Madison-Antenucci S, Woolford JL Jr, Tong L, Frank J. Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit. Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12174-12179. Epub 2016 Oct 10. PMID:27791004 doi:http://dx.doi.org/10.1073/pnas.1614594113

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5t5h"

@@ Line 3: / Line 3: @@
 <SX load='5t5h' size='340' side='right' viewer='molstar' caption='[[5t5h]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5t5h]] is a 46 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T5H FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5t5h]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T5H FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.54&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=A2M:2-O-METHYLADENOSINE+5-(DIHYDROGEN+PHOSPHATE)'>A2M</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=A2M:2-O-METHYLADENOSINE+5-(DIHYDROGEN+PHOSPHATE)'>A2M</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5h OCA], [http://pdbe.org/5t5h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t5h RCSB], [http://www.ebi.ac.uk/pdbsum/5t5h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5h ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5h OCA], [https://pdbe.org/5t5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t5h RCSB], [https://www.ebi.ac.uk/pdbsum/5t5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5h ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/Q4DXW6_TRYCC Q4DXW6_TRYCC]] Binds to the 23S rRNA.[RuleBase:RU000576]
+[https://www.uniprot.org/uniprot/RL18_TRYCC RL18_TRYCC]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 28: / Line 28: @@
 [[Category: Large Structures]]
 [[Category: Trypanosoma cruzi]]
-[[Category: Espina, N]]
+[[Category: Espina N]]
-[[Category: Frank, J]]
+[[Category: Frank J]]
-[[Category: Grassucci, R A]]
+[[Category: Grassucci RA]]
-[[Category: Gutierrez-Vargas, C]]
+[[Category: Gutierrez-Vargas C]]
-[[Category: Liu, Z]]
+[[Category: Liu Z]]
-[[Category: Madison-Antenucci, S]]
+[[Category: Madison-Antenucci S]]
-[[Category: Ramesh, M]]
+[[Category: Ramesh M]]
-[[Category: Sun, M]]
+[[Category: Sun M]]
-[[Category: Tong, L]]
+[[Category: Tong L]]
-[[Category: Tutuncuoglu, B]]
+[[Category: Tutuncuoglu B]]
-[[Category: Wei, J]]
+[[Category: Wei J]]
-[[Category: Woolford, J L]]
+[[Category: Woolford Jr JL]]
-[[Category: 5 angstrom cryo-em structure]]
-[[Category: Assmebly]]
-[[Category: Multiply fragmented rrna]]
-[[Category: Ribosomal subunit]]
-[[Category: Ribosome]]

5t5h

From Proteopedia

Current revision

Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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