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1uxs

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[[Image:1uxs.gif|left|200px]]
[[Image:1uxs.gif|left|200px]]
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{{Structure
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|PDB= 1uxs |SIZE=350|CAPTION= <scene name='initialview01'>1uxs</scene>, resolution 1.55&Aring;
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{{STRUCTURE_1uxs| PDB=1uxs | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxs OCA], [http://www.ebi.ac.uk/pdbsum/1uxs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uxs RCSB]</span>
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'''CRYSTAL STRUCTURE OF HLA-B*2705 COMPLEXED WITH THE LATENT MEMBRANE PROTEIN 2 PEPTIDE (LMP2)OF EPSTEIN-BARR VIRUS'''
'''CRYSTAL STRUCTURE OF HLA-B*2705 COMPLEXED WITH THE LATENT MEMBRANE PROTEIN 2 PEPTIDE (LMP2)OF EPSTEIN-BARR VIRUS'''
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[[Category: Uchanska-Ziegler, B.]]
[[Category: Uchanska-Ziegler, B.]]
[[Category: Ziegler, A.]]
[[Category: Ziegler, A.]]
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[[Category: epstein-barr virus,]]
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[[Category: Epstein-barr virus]]
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[[Category: hla-b*2705]]
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[[Category: Hla-b*2705]]
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[[Category: immune system]]
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[[Category: Immune system]]
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[[Category: mhc (major histocompatibility complex)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:49:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:16:49 2008''
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Revision as of 08:49, 3 May 2008

Template:STRUCTURE 1uxs

CRYSTAL STRUCTURE OF HLA-B*2705 COMPLEXED WITH THE LATENT MEMBRANE PROTEIN 2 PEPTIDE (LMP2)OF EPSTEIN-BARR VIRUS


Overview

Molecular mimicry is discussed as a possible mechanism that may contribute to the development of autoimmune diseases. It could also be involved in the differential association of the human major histocompatibility subtypes HLA-B(*)2705 and HLA-B(*)2709 with ankylosing spondylitis. These two subtypes differ only in residue 116 of the heavy chain (Asp in B(*)2705 and His in B(*)2709), but the reason for the differential disease association is not understood. Using x-ray crystallography, we show here that the viral peptide pLMP2 (RRRWRRLTV, derived from latent membrane protein 2 (residues 236-244) of Epstein-Barr virus) is presented by the B(*)2705 and B(*)2709 molecules in two drastically deviating conformations. Extensive structural similarity between pLMP2 and the self-peptide pVIPR (RRKWRRWHL, derived from vasoactive intestinal peptide type 1 receptor (residues 400-408)) is observed only when the peptides are presented by B(*)2705 because of a salt bridge between Arg(5) of both peptides and the subtype-specific heavy chain residue Asp(116). Combined with functional studies using pLMP2/pVIPR-cross-reactive cytotoxic T cell lines and clones, together with target cells presenting these peptides or a modified peptide analogue, our results reveal that a pathogen-derived peptide can exhibit major histocompatibility complex class I subtype-dependent, drastically distinct binding modes. Furthermore, the results demonstrate that molecular mimicry between pLMP2 and pVIPR in the HLA-B27 context is an allele-dependent property.

About this Structure

1UXS is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Allele-dependent similarity between viral and self-peptide presentation by HLA-B27 subtypes., Fiorillo MT, Ruckert C, Hulsmeyer M, Sorrentino R, Saenger W, Ziegler A, Uchanska-Ziegler B, J Biol Chem. 2005 Jan 28;280(4):2962-71. Epub 2004 Nov 10. PMID:15537660 Page seeded by OCA on Sat May 3 11:49:52 2008

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