Cocaine esterase
From Proteopedia
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| - | < | + | <StructureSection load='1l7q' size='350' side='right' caption='Cocaine esterase complex with reaction product benzoic acid (PDB entry [[1l7q]])' scene='48/489298/Cv/1'> |
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| - | ''' | + | |
| + | __TOC__ | ||
| + | == Function == | ||
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| + | '''Cocaine esterase''' (CocE) catalyzes the hydrolysis of the benzoyl ester of cocaine to ecgonine methyl ester and benzoic acid. CocE is produced by bacteria which thrive in soil of cocaine-producing plants. These bacteria use cocaine as their sole source of carbon and nitrogen. <ref>PMID:11742345</ref> | ||
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| + | == Relevance == | ||
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| + | CocE is being investigated as a possible therapeutic agent for treatment of cocaine abuse. | ||
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| + | == Structural highlights == | ||
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| + | CocE contains 3 domains. <scene name='48/489298/Cv/10'>Domain 1</scene> contains the catalytic triad: Ser, Asp and His typical of the hydrolase family. <scene name='48/489298/Cv/11'>Domains 2 and 3</scene> are involved in substrate recognition. <scene name='48/489298/Cv/12'>The active site</scene> is located at a cleft in the interface of the 3 domains and contains the <scene name='48/489298/Cv/13'>catalytic triad Ser-Asp-His</scene>. | ||
| + | </StructureSection> | ||
==3D structures of cocaine esterase== | ==3D structures of cocaine esterase== | ||
| - | [[3i2j]], [[3puh]] - RhCocE – ''Rhodococcus''<br /> | + | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} |
| - | [[1l7r]], [[3pui]] - RhCocE (mutant) | + | {{#tree:id=OrganizedByTopic|openlevels=0| |
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| + | *Cocaine esterase | ||
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| + | **[[3i2j]], [[3puh]] - RhCocE – ''Rhodococcus''<br /> | ||
| + | **[[1l7r]], [[3pui]], [[4p08]], [[9k48]] - RhCocE (mutant) | ||
| - | + | *Cocaine esterase binary complex | |
| - | [[1ju3]] – RhCocE + transition state inhibitor <br /> | + | **[[1ju3]] – RhCocE + transition state inhibitor <br /> |
| - | [[1ju4]] – RhCocE + benzoic acid<br /> | + | **[[1ju4]] – RhCocE + benzoic acid<br /> |
| - | [[1l7q]] - RhCocE (mutant) + benzoic acid<br /> | + | **[[1l7q]], [[7f65]] - RhCocE (mutant) + benzoic acid<br /> |
| - | [[3ida]], [[3i2f]], [[3i2g]], [[3i2h]], [[3i2i]] - RhCocE (mutant) + DTT derivative<br /> | + | **[[3ida]], [[3i2f]], [[3i2g]], [[3i2h]], [[3i2i]] - RhCocE (mutant) + DTT derivative<br /> |
| - | [[3i2k]] - RhCocE + DTT derivative | + | **[[3i2k]] - RhCocE + DTT derivative |
| + | }} | ||
| + | == References == | ||
| + | <references/> | ||
| + | [[Category: Topic Page]] | ||
Current revision
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3D structures of cocaine esterase
Updated on 02-February-2025
References
- ↑ Larsen NA, Turner JM, Stevens J, Rosser SJ, Basran A, Lerner RA, Bruce NC, Wilson IA. Crystal structure of a bacterial cocaine esterase. Nat Struct Biol. 2002 Jan;9(1):17-21. PMID:11742345 doi:10.1038/nsb742
