1v2x

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[[Image:1v2x.jpg|left|200px]]
[[Image:1v2x.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1v2x |SIZE=350|CAPTION= <scene name='initialview01'>1v2x</scene>, resolution 1.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1v2x", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA_guanosine-2'-O-methyltransferase tRNA guanosine-2'-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.34 2.1.1.34] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1v2x| PDB=1v2x | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2x OCA], [http://www.ebi.ac.uk/pdbsum/1v2x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1v2x RCSB]</span>
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}}
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'''TrmH'''
'''TrmH'''
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: tRNA guanosine-2'-O-methyltransferase]]
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[[Category: TRNA guanosine-2'-O-methyltransferase]]
[[Category: Endo, Y.]]
[[Category: Endo, Y.]]
[[Category: Fukai, S.]]
[[Category: Fukai, S.]]
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[[Category: Watanabe, K.]]
[[Category: Watanabe, K.]]
[[Category: Yokoyama, S.]]
[[Category: Yokoyama, S.]]
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[[Category: deep trefoil knot]]
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[[Category: Deep trefoil knot]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: Rsgi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:00:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:18:44 2008''
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Revision as of 09:00, 3 May 2008

Image:1v2x.jpg

Template:STRUCTURE 1v2x

TrmH


Overview

The tRNA(Gm18) methyltransferase (TrmH) catalyzes the 2'-O methylation of guanosine 18 (Gua18) of tRNA. We solved the crystal structure of Thermus thermophilus TrmH complexed with S-adenosyl-L-methionine at 1.85 A resolution. The catalytic domain contains a deep trefoil knot, which mutational analyses revealed to be crucial for the formation of the catalytic site and the cofactor binding pocket. The tRNA dihydrouridine(D)-arm can be docked onto the dimeric TrmH, so that the tRNA D-stem is clamped by the N- and C-terminal helices from one subunit while the Gua18 is modified by the other subunit. Arg41 from the other subunit enters the catalytic site and forms a hydrogen bond with a bound sulfate ion, an RNA main chain phosphate analog, thus activating its nucleophilic state. Based on Gua18 modeling onto the active site, we propose that once Gua18 binds, the phosphate group activates Arg41, which then deprotonates the 2'-OH group for methylation.

About this Structure

1V2X is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Deep knot structure for construction of active site and cofactor binding site of tRNA modification enzyme., Nureki O, Watanabe K, Fukai S, Ishii R, Endo Y, Hori H, Yokoyama S, Structure. 2004 Apr;12(4):593-602. PMID:15062082 Page seeded by OCA on Sat May 3 12:00:40 2008

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