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| | ==Crystal structure of samarosporin I at 100K== | | ==Crystal structure of samarosporin I at 100K== |
| - | <StructureSection load='4g13' size='340' side='right' caption='[[4g13]], [[Resolution|resolution]] 0.80Å' scene=''> | + | <StructureSection load='4g13' size='340' side='right'caption='[[4g13]], [[Resolution|resolution]] 0.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4g13]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Samarospora_rostrup Samarospora rostrup]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G13 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G13 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4g13]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Samarospora_rostrup Samarospora rostrup]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G13 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AIB:ALPHA-AMINOISOBUTYRIC+ACID'>AIB</scene>, <scene name='pdbligand=DIV:D-ISOVALINE'>DIV</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=PHL:L-PHENYLALANINOL'>PHL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1joh|1joh]], [[1ob4|1ob4]], [[1ob6|1ob6]], [[1ob7|1ob7]], [[4g14|4g14]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AIB:ALPHA-AMINOISOBUTYRIC+ACID'>AIB</scene>, <scene name='pdbligand=DIV:D-ISOVALINE'>DIV</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=PHL:L-PHENYLALANINOL'>PHL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g13 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g13 RCSB], [http://www.ebi.ac.uk/pdbsum/4g13 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g13 OCA], [https://pdbe.org/4g13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g13 RCSB], [https://www.ebi.ac.uk/pdbsum/4g13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g13 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4g13" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Samarospora rostrup]] | | [[Category: Samarospora rostrup]] |
| - | [[Category: Axford, D]] | + | [[Category: Axford D]] |
| - | [[Category: Gessmann, R]] | + | [[Category: Gessmann R]] |
| - | [[Category: Petratos, K]] | + | [[Category: Petratos K]] |
| - | [[Category: Antibiotic]]
| + | |
| - | [[Category: Antibiotic peptide]]
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| - | [[Category: Extracellular]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Peptaibol]]
| + | |
| Structural highlights
4g13 is a 1 chain structure with sequence from Samarospora rostrup. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 0.8Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
The atomic resolution structures of samarosporin I have been determined at 100 and 293 K. This is the first crystal structure of a natural 15-residue peptaibol. The amino acid sequence in samarosporin I is identical to emerimicin IV and stilbellin I. Samarosporin is a peptide antibiotic produced by the ascomycetous fungus Samarospora rostrup and belongs to peptaibol subfamily 2. The structures at both temperatures are very similar to each other adopting mainly a 3(10) -helical and a minor fraction of alpha-helical conformation. The helices are significantly bent and packed in an antiparallel fashion in the centered monoclinic lattice leaving among them an approximately 10-A channel extending along the crystallographic twofold axis. Only two ordered water molecules per peptide molecule were located in the channel. Comparisons have been carried out with crystal structures of subfamily 2 16-residue peptaibols antiamoebin and cephaibols. The repercussion of the structural analysis of samarosporin on membrane function is discussed. Copyright (c) 2012 European Peptide Society and John Wiley & Sons, Ltd.
The crystal structure of samarosporin I at atomic resolution.,Gessmann R, Axford D, Evans G, Bruckner H, Petratos K J Pept Sci. 2012 Nov;18(11):678-84. doi: 10.1002/psc.2454. Epub 2012 Sep 28. PMID:23019149[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gessmann R, Axford D, Evans G, Bruckner H, Petratos K. The crystal structure of samarosporin I at atomic resolution. J Pept Sci. 2012 Nov;18(11):678-84. doi: 10.1002/psc.2454. Epub 2012 Sep 28. PMID:23019149 doi:http://dx.doi.org/10.1002/psc.2454
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