1vfg
From Proteopedia
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'''Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog''' | '''Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog''' | ||
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[[Category: Ueda, T.]] | [[Category: Ueda, T.]] | ||
[[Category: Vassylyev, D G.]] | [[Category: Vassylyev, D G.]] | ||
| - | [[Category: | + | [[Category: Riken structural genomics/proteomics initiative]] |
| - | [[Category: | + | [[Category: Rna]] |
| - | [[Category: | + | [[Category: Rsgi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Transferase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:28:48 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:28, 3 May 2008
Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog
Overview
The 3'-terminal CCA nucleotide sequence (positions 74-76) of transfer RNA is essential for amino acid attachment and interaction with the ribosome during protein synthesis. The CCA sequence is synthesized de novo and/or repaired by a template-independent RNA polymerase, 'CCA-adding enzyme', using CTP and ATP as substrates. Despite structural and biochemical studies, the mechanism by which the CCA-adding enzyme synthesizes the defined sequence without a nucleic acid template remains elusive. Here we present the crystal structure of Aquifex aeolicus CCA-adding enzyme, bound to a primer tRNA lacking the terminal adenosine and an incoming ATP analogue, at 2.8 A resolution. The enzyme enfolds the acceptor T helix of the tRNA molecule. In the catalytic pocket, C75 is adjacent to ATP, and their base moieties are stacked. The complementary pocket for recognizing C74-C75 of tRNA forms a 'protein template' for the penultimate two nucleotides, mimicking the nucleotide template used by template-dependent polymerases. These results are supported by systematic analyses of mutants. Our structure represents the 'pre-insertion' stage of selecting the incoming nucleotide and provides the structural basis for the mechanism underlying template-independent RNA polymerization.
About this Structure
1VFG is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Structural basis for template-independent RNA polymerization., Tomita K, Fukai S, Ishitani R, Ueda T, Takeuchi N, Vassylyev DG, Nureki O, Nature. 2004 Aug 5;430(7000):700-4. PMID:15295603 Page seeded by OCA on Sat May 3 12:28:48 2008
Categories: Aquifex aeolicus | Polynucleotide adenylyltransferase | Single protein | Fukai, S. | Ishitani, R. | Nureki, O. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Takeuchi, N. | Tomita, K. | Ueda, T. | Vassylyev, D G. | Riken structural genomics/proteomics initiative | Rna | Rsgi | Structural genomic | Transferase
