Lactoperoxidase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:30, 2 April 2025) (edit) (undo)
 
(31 intermediate revisions not shown.)
Line 1: Line 1:
-
<StructureSection load='3eri' size='350' side='right' caption='Glycosylated bovine lactoferrin containing heme complex with thiocyanate, I- (purple) and Ca+2 (green) ions (PDB entry [[3eri]])' scene=''>
+
<StructureSection load='3eri' size='350' side='right' caption='Glycosylated bovine lactoferrin containing heme complex with thiocyanate, I- (purple) and Ca+2 (green) ions (PDB entry [[3eri]])' scene='48/487283/Cv/1'>
== Function ==
== Function ==
'''Lactoperoxidase''' (LPO) catalyzes the oxidation of thiocyanate, bromide and iodide using hydrogen peroxide. LPO is the second most abundant enzyme in milk. Heme is the cofactor of LPO. LPO contains a strongly-chelated calcium ion<ref>PMID:10837362</ref>.
'''Lactoperoxidase''' (LPO) catalyzes the oxidation of thiocyanate, bromide and iodide using hydrogen peroxide. LPO is the second most abundant enzyme in milk. Heme is the cofactor of LPO. LPO contains a strongly-chelated calcium ion<ref>PMID:10837362</ref>.
Line 7: Line 7:
== Structural highlights ==
== Structural highlights ==
-
The peroxidation of thiocyanate catalyzed by LPO occurs at the heme cavity site<ref>PMID:19339248</ref>.
+
The <scene name='48/487283/Cv/6'>peroxidation of thiocyanate catalyzed by LPO occurs at the heme cavity site</scene><ref>PMID:19339248</ref>. Water molecules shown as red spheres. <scene name='48/487283/Cv/7'>Whole active site</scene>.
</StructureSection>
</StructureSection>
Line 18: Line 18:
**[[2pt3]] - bPLO + phosphate - bovine<br />
**[[2pt3]] - bPLO + phosphate - bovine<br />
-
**[[2nqx]] – bPLO + I<br />
+
**[[2nqx]], [[5b72]] – bPLO + I<br />
 +
**[[7dlq]] – bPLO + H2O2<br />
**[[2r5l]] – gLPO + I – goat<br />
**[[2r5l]] – gLPO + I – goat<br />
**[[3nak]] – gLPO + formate<br />
**[[3nak]] – gLPO + formate<br />
-
**[[4ig5]], [[4qyq]] – gLPO + antithyroid agent<br />
+
**[[6ky7]], [[7c73]], [[7c74]], [[7c75]], [[7d52]], [[7dao]], [[7de5]], [[7dmr]] – yPLO + Ca - yak<br />
 +
**[[7c74]] - yLPO + Cl <br />
 +
**[[6l32]], [[6l42]], [[7d52]], [[6l5g]], [[7c75]] - yLPO + Zn <br />
 +
**[[6lqw]], [[7y3u]] – yPLO + H2O2<br />
 +
**[[7c73]] – yPLO + phosphoserine<br />
*Lactoperoxidase ternary complex
*Lactoperoxidase ternary complex
-
**[[2gj1]] – bLPO + carbonate + nitrate <br />
+
**[[2ips]], [[3eri]], [[3gc1]], [[6a4y]], [[9m0i]], [[6m7e]], [[7dn7]] – bLPO + SCN + I<br />
-
**[[2ips]], [[3eri]], [[3gc1]] – bLPO + SCN + I<br />
+
**[[2qrb]] – bLPO + SCN + Cl<br />
**[[2qrb]] – bLPO + SCN + Cl<br />
**[[3bxi]] – bLPO + SCN + nitrate<br />
**[[3bxi]] – bLPO + SCN + nitrate<br />
**[[4pnx]] - bPLO + bromomethane + I<br />
**[[4pnx]] - bPLO + bromomethane + I<br />
**[[2pum]] – bPLO + catechol + I<br />
**[[2pum]] – bPLO + catechol + I<br />
-
**[[2gj1]] – bLPO + carbonate + nitrate <br />
 
**[[3krq]] – bLPO + amino-triazole + I<br />
**[[3krq]] – bLPO + amino-triazole + I<br />
 +
**[[6kmk]] – bPLO + H2O2 + phosphoserine<br />
 +
**[[7dlq]], [[7dn6]] – bPLO + H2O2 + I<br />
**[[2z5z]] – wbPLO + F + I – water buffalo<br />
**[[2z5z]] – wbPLO + F + I – water buffalo<br />
**[[2o86]] – wbPLO + nitrate + I<br />
**[[2o86]] – wbPLO + nitrate + I<br />
**[[3erh]] - wbLPO + SCN + I<br />
**[[3erh]] - wbLPO + SCN + I<br />
 +
**[[7de5]] - yLPO + SCN + I<br />
**[[2ojv]] – gPLO + CN + I<br />
**[[2ojv]] – gPLO + CN + I<br />
**[[3n8f]] - gLPO + SCN + nitrate<br />
**[[3n8f]] - gLPO + SCN + nitrate<br />
 +
**[[5ff1]] - gLPO + methimazole + nitrate<br />
 +
**[[5hpw]] - gLPO + propyl-thiouracil + nitrate<br />
**[[3niu]] – gLPO + diethylene glycol + phosphate<br />
**[[3niu]] – gLPO + diethylene glycol + phosphate<br />
**[[3rke]] - gPLO + inhibitor + I<br />
**[[3rke]] - gPLO + inhibitor + I<br />
-
**[[2ikc]] – sLPO + carbonate + formate – sheep
+
**[[2ikc]] – sLPO + carbonate + formate – sheep<br />
 +
**[[7ve3]] - sLPO + hypoiodite + I<br />
*Lactoperoxidase quaternary complex
*Lactoperoxidase quaternary complex
Line 50: Line 59:
**[[3i6n]] - bPLO + isoniazid + SCN + I<br />
**[[3i6n]] - bPLO + isoniazid + SCN + I<br />
**[[4ksz]] - bPLO + cystein + SCN + I<br />
**[[4ksz]] - bPLO + cystein + SCN + I<br />
-
**[[3nyh]] - bPLO + Br + Cl + SCN + I<br />
+
**[[5wv3]] - bPLO + oxidosulfanyl-methanamine + Br + I<br />
-
**[[3ogw]] - bPLO + indomethacin + SCN + I<br />
+
**[[7dlq]] - bPLO + Br + Cl + SCN + I<br />
-
**[[3py4]], [[4gn6]] - bPLO + paracetamol + SCN + I<br />
+
**[[7wyj]] - bPLO + indomethacin + SCN + I<br />
-
**[[3q9k]] - bPLO + phenyliso-SCN + SCN + I<br />
+
**[[3py4]], [[6m7e]] - bPLO + paracetamol + SCN + I<br />
-
**[[3ql6]] - bPLO + nitro-phenoxymethanesulfonanilide+ SCN + I<br />
+
**[[7dn6]] - bPLO + phenyliso-SCN + SCN + I<br />
-
**[[3s4f]], [[4njb]], [[4nt3]] - bPLO + pyridine derivative + SCN + I<br />
+
**[[7dn7]] - bPLO + nitro-phenoxymethanesulfonanilide+ SCN + I<br />
-
**[[3r4x]], [[3tuw]] - bPLO + pyrazine derivative + SCN + I<br />
+
**[[8y9x]], [[4njb]], [[9m0i]] - bPLO + pyridine derivative + SCN + I<br />
 +
**[[3r4x]], [[5zgs]] - bPLO + pyrazine derivative + SCN + I<br />
**[[3r5o]] - bPLO + methoxyphenol + SCN + I<br />
**[[3r5o]] - bPLO + methoxyphenol + SCN + I<br />
**[[3tgy]] - bPLO + ascorbate + SCN + I<br />
**[[3tgy]] - bPLO + ascorbate + SCN + I<br />
**[[3v6q]] - bPLO + CO + SCN + I<br />
**[[3v6q]] - bPLO + CO + SCN + I<br />
 +
**[[6l9t]] - bLPO + Zn + OSCN + I<br />
 +
**[[5gls]] - bLPO + butyl-triazole + SCN + I<br />
 +
**[[5gh0]] - bLPO + mercaptoimidazole + nitrate + carbonate<br />
**[[2gjm]] - wbPLO + carbonate + SCN + I<br />
**[[2gjm]] - wbPLO + carbonate + SCN + I<br />
**[[3faq]] – wbLPO + CN + SCN + I<br />
**[[3faq]] – wbLPO + CN + SCN + I<br />
**[[3fnl]] - wbPLO + salicylhydroxamic acid + SCN + I<br />
**[[3fnl]] - wbPLO + salicylhydroxamic acid + SCN + I<br />
 +
**[[4y55]] - wbLPO + rhodanide + SCN + I<br />
**[[2e9e]] – gPLO + nitrate + carbonate + SCN + CN<br />
**[[2e9e]] – gPLO + nitrate + carbonate + SCN + CN<br />
**[[2efb]] – gPLO + phosphate + carbonate + SCN + CN<br />
**[[2efb]] – gPLO + phosphate + carbonate + SCN + CN<br />
**[[3qf1]] - gPLO + piperazine + SCN + I<br />
**[[3qf1]] - gPLO + piperazine + SCN + I<br />
-
**[[4ljj]] - gPLO + acrylonitrile + SCN + I<br />
+
**[[8ing]] - gPLO + acrylonitrile + SCN + I<br />
**[[4msf]], [[4oek]] - gPLO + phenol derivative+ SCN + I<br />
**[[4msf]], [[4oek]] - gPLO + phenol derivative+ SCN + I<br />
**[[2eha]] – gPLO + formate + carbonate + SCN<br />
**[[2eha]] – gPLO + formate + carbonate + SCN<br />
-
**[[3r55]], [[4n7a]] - gPLO + pyrazine derivative + SCN + I<br />
+
**[[3r55]], [[6lf7]] - gPLO + pyrazine derivative + SCN + I<br />
**[[3sxv]] - gPLO + amitrole + SCN + I<br />
**[[3sxv]] - gPLO + amitrole + SCN + I<br />
-
**[[3r5q]] - sPLO +tetrahydrofuran + SCN + I<br />
 
**[[4qjq]] - gPLO + octopamine + SCN + I<br />
**[[4qjq]] - gPLO + octopamine + SCN + I<br />
-
**[[4s0y]] - bLPO + butyl-triazole + SCN + I<br />
+
**[[6lf7]] - gPLO + hypocyanite + SCN + H2O2 + I<br />
-
**[[4y55]] - wbLPO + rhodanide + SCN + I<br />
+
**[[7ve3]] - sPLO +tetrahydrofuran + SCN + I<br />
 +
**[[6ky7]], [[6l2j]] – yPLO + H2O2 + phosphoserine + SCN + I<br />
}}
}}

Current revision

Glycosylated bovine lactoferrin containing heme complex with thiocyanate, I- (purple) and Ca+2 (green) ions (PDB entry 3eri)

Drag the structure with the mouse to rotate

3D structures of lactoperoxidase

Updated on 02-April-2025

References

  1. ↑ Gerson C, Sabater J, Scuri M, Torbati A, Coffey R, Abraham JW, Lauredo I, Forteza R, Wanner A, Salathe M, Abraham WM, Conner GE. The lactoperoxidase system functions in bacterial clearance of airways. Am J Respir Cell Mol Biol. 2000 Jun;22(6):665-71. PMID:10837362
  2. ↑ Gerson C, Sabater J, Scuri M, Torbati A, Coffey R, Abraham JW, Lauredo I, Forteza R, Wanner A, Salathe M, Abraham WM, Conner GE. The lactoperoxidase system functions in bacterial clearance of airways. Am J Respir Cell Mol Biol. 2000 Jun;22(6):665-71. PMID:10837362
  3. ↑ Sheikh IA, Singh AK, Singh N, Sinha M, Singh SB, Bhushan A, Kaur P, Srinivasan A, Sharma S, Singh TP. Structural evidence of substrate specificity in mammalian peroxidases: structure of the thiocyanate complex with lactoperoxidase and its interactions at 2.4 A resolution. J Biol Chem. 2009 May 29;284(22):14849-56. Epub 2009 Apr 1. PMID:19339248 doi:10.1074/jbc.M807644200

Proteopedia Page Contributors and Editors (what is this?)

Joel L. Sussman, Michal Harel, Alexander Berchansky, Jaime Prilusky

Retrieved from "http://52.214.119.220/wiki/index.php/Lactoperoxidase"
Personal tools