2bhs

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[[Image:2bhs.gif|left|200px]]<br />
 
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<applet load="2bhs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bhs, resolution 2.67&Aring;" />
 
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'''CRYSTAL STRUCTURE OF CYSTEINE SYNTHASE B'''<br />
 
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==Overview==
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==Crystal Structure of Cysteine Synthase B==
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The enzyme O-acetylserine sulfhydrylase participates in the biosynthesis, of l-cysteine in bacteria and plants. The structure of isoenzyme B (CysM), from Escherichia coli was established in a hexagonal crystal form at 2.7 A, resolution (wild-type) and in a merohedrally twinned tetragonal crystal, form at 2.1 A resolution (surface mutant). Structural superpositions, revealed the variations with respect to isoenzyme A (CysK) and explained, the different substrate specificities. A geometric model of the reaction, catalyzed by CysM is proposed. Both isoenzymes are used for the production, of l-amino acid derivatives as building blocks for the synthesis of, peptides and peptidomimetic drugs. Since the structure of CysM revealed a, remarkable main chain variation at the active center, it ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15952768 (full description)]]
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<StructureSection load='2bhs' size='340' side='right'caption='[[2bhs]], [[Resolution|resolution]] 2.67&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bhs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BHS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.67&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bhs OCA], [https://pdbe.org/2bhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bhs RCSB], [https://www.ebi.ac.uk/pdbsum/2bhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bhs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYSM_ECOLI CYSM_ECOLI] Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/2bhs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bhs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The enzyme O-acetylserine sulfhydrylase participates in the biosynthesis of l-cysteine in bacteria and plants. The structure of isoenzyme B (CysM) from Escherichia coli was established in a hexagonal crystal form at 2.7 A resolution (wild-type) and in a merohedrally twinned tetragonal crystal form at 2.1 A resolution (surface mutant). Structural superpositions revealed the variations with respect to isoenzyme A (CysK) and explained the different substrate specificities. A geometric model of the reaction catalyzed by CysM is proposed. Both isoenzymes are used for the production of l-amino acid derivatives as building blocks for the synthesis of peptides and peptidomimetic drugs. Since the structure of CysM revealed a remarkable main chain variation at the active center, it constitutes a further starting point for engineering mutants with novel substrate specificities.
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==About this Structure==
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Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli.,Claus MT, Zocher GE, Maier TH, Schulz GE Biochemistry. 2005 Jun 21;44(24):8620-6. PMID:15952768<ref>PMID:15952768</ref>
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2BHS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with PLP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Cysteine_synthase Cysteine synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.47 2.5.1.47]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BHS OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli., Claus MT, Zocher GE, Maier TH, Schulz GE, Biochemistry. 2005 Jun 21;44(24):8620-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15952768 15952768]
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</div>
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[[Category: Cysteine synthase]]
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<div class="pdbe-citations 2bhs" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Claus, M.T.]]
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[[Category: Claus MT]]
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[[Category: Maier, T.H.P.]]
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[[Category: Maier THP]]
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[[Category: Schulz, G.E.]]
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[[Category: Schulz GE]]
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[[Category: Zocher, G.E.]]
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[[Category: Zocher GE]]
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[[Category: PLP]]
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[[Category: cysteine biosynthesis]]
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[[Category: plp-dependent enzyme]]
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[[Category: pyridoxal phosphate]]
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[[Category: pyridoxal-5'-phosphate]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:38:13 2007''
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Current revision

Crystal Structure of Cysteine Synthase B

PDB ID 2bhs

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