1vq2
From Proteopedia
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| - | + | {{STRUCTURE_1vq2| PDB=1vq2 | SCENE= }} | |
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'''CRYSTAL STRUCTURE OF T4-BACTERIOPHAGE DEOXYCYTIDYLATE DEAMINASE, MUTANT R115E''' | '''CRYSTAL STRUCTURE OF T4-BACTERIOPHAGE DEOXYCYTIDYLATE DEAMINASE, MUTANT R115E''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1VQ2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. This structure supersedes the now removed PDB entry | + | 1VQ2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1teo 1teo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQ2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Enterobacteria phage t4]] | [[Category: Enterobacteria phage t4]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: | + | [[Category: DCMP deaminase]] |
[[Category: Almog, R.]] | [[Category: Almog, R.]] | ||
[[Category: Maccoll, R.]] | [[Category: Maccoll, R.]] | ||
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[[Category: Maley, G F.]] | [[Category: Maley, G F.]] | ||
[[Category: Roey, P Van.]] | [[Category: Roey, P Van.]] | ||
| - | [[Category: | + | [[Category: Hydrolase]] |
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Revision as of 09:47, 3 May 2008
CRYSTAL STRUCTURE OF T4-BACTERIOPHAGE DEOXYCYTIDYLATE DEAMINASE, MUTANT R115E
Overview
2'-Deoxycytidylate deaminase (dCD) converts deoxycytidine 5'-monophosphate (dCMP) to deoxyuridine 5'-monophosphate and is a major supplier of the substrate for thymidylate synthase, an important enzyme in DNA synthesis and a major target for cancer chemotherapy. Wild-type dCD is allosterically regulated by the end products of its metabolic pathway, deoxycytidine 5'-triphosphate and deoxythymidine 5'-triphosphate, which act as an activator and an inhibitor, respectively. The first crystal structure of a dCD, in the form of the R115E mutant of the T4-bacteriophage enzyme complexed with the active site inhibitor pyrimidin-2-one deoxyribotide, has been determined at 2.2 A resolution. This mutant of dCD is active, even in the absence of the allosteric regulators. The molecular topology of dCD is related to that of cytidine deaminase (CDA) but with modifications for formation of the binding site for the phosphate group of dCMP. The enzyme has a zinc ion-based mechanism that is similar to that of CDA. A second zinc ion that is present in bacteriophage dCD, but absent in mammalian dCD and CDA, is important for the structural integrity of the enzyme and for the binding of the phosphate group of the substrate or inhibitor. Although the R115E mutant of dCD is a dimer in solution, it crystallizes as a hexamer, mimicking the natural state of the wild-type enzyme. Residues 112 and 115, which are known to be important for the binding of the allosteric regulators, are found in a pocket that is at the intersubunit interfaces in the hexamer but distant from the substrate-binding site. The substrate-binding site is composed of residues from a single protein molecule and is sequestered in a deep groove. This groove is located at the outer surface of the hexamer but ends at the subunit interface that also includes residue 115. It is proposed that the absence of subunit interactions at this interface in the dimeric R115E mutant renders the substrate-binding site accessible. In contrast, for the wild-type enzyme, binding of dCTP induces an allosteric effect that affects the subunit interactions and results in an increase in the accessibility of the binding site.
About this Structure
1VQ2 is a Single protein structure of sequence from Enterobacteria phage t4. This structure supersedes the now removed PDB entry 1teo. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the R115E mutant of T4-bacteriophage 2'-deoxycytidylate deaminase., Almog R, Maley F, Maley GF, Maccoll R, Van Roey P, Biochemistry. 2004 Nov 2;43(43):13715-23. PMID:15504034 Page seeded by OCA on Sat May 3 12:46:59 2008
