9hnl
From Proteopedia
(Difference between revisions)
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- | '''Unreleased structure''' | ||
- | + | ==Structure of the (6-4) photolyase of Caulobacter crescentus in its oxidized state at room temperture-synchrotron== | |
+ | <StructureSection load='9hnl' size='340' side='right'caption='[[9hnl]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[9hnl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9HNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9HNL FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DLZ:1-DEOXY-1-(6,7-DIMETHYL-2,4-DIOXO-3,4-DIHYDROPTERIDIN-8(2H)-YL)-D-RIBITOL'>DLZ</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9hnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9hnl OCA], [https://pdbe.org/9hnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9hnl RCSB], [https://www.ebi.ac.uk/pdbsum/9hnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9hnl ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/Q9AAF5_CAUVC Q9AAF5_CAUVC] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Photolyases repair UV damage to DNA by using absorbed blue light. Within the photolyase/cryptochrome superfamily (PCSf), a major subgroup consists of prokaryotic (6-4) photolyases. These enzymes rely on flavin adenine dinucleotide (FAD) as a catalytic cofactor, besides an ancillary antenna chromophore, and a [4Fe-4S] cluster with yet unknown function. For the prokaryotic 6-4 photolyase of Caulobacter crescentus, we investigated structural changes associated with its different redox states by damage-free crystallography using X-ray free-electron lasers. EPR and optical spectroscopy confirmed redox-dependent structural transitions, including the formation of an oxidized [4Fe-4S](3+) cluster with the dynamic cleavage of a single iron-sulfur bond. Photoreduction to the catalytic FADH(-) state alters the flavin binding site at the proximal aromatic pair Y390/F394 that is part of the electron transport pathway. Upon oxidation, the observable structural transitions of the protein matrix around the [4Fe-4S] cluster may affect DNA binding and are consistent with the much-debated role of the iron-sulfur cluster in DNA-binding proteins for quenching electron holes. | ||
- | + | Redox-State-Dependent Structural Changes within a Prokaryotic 6-4 Photolyase.,Wang PH, Hosokawa Y, C Soares J, Emmerich HJ, Fuchs V, Caramello N, Engilberge S, Bologna A, Rosner CJ, Nakamura M, Watad M, Luo F, Owada S, Tosha T, Kang J, Tono K, Bessho Y, Nango E, Pierik AJ, Royant A, Tsai MD, Yamamoto J, Maestre-Reyna M, Essen LO J Am Chem Soc. 2025 Apr 29. doi: 10.1021/jacs.4c18116. PMID:40298610<ref>PMID:40298610</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
- | [[Category: Essen | + | <div class="pdbe-citations 9hnl" style="background-color:#fffaf0;"></div> |
- | [[Category: Maestre-Reyna | + | == References == |
- | [[Category: Po Hsun | + | <references/> |
+ | __TOC__ | ||
+ | </StructureSection> | ||
+ | [[Category: Caulobacter vibrioides]] | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Essen L-O]] | ||
+ | [[Category: Maestre-Reyna M]] | ||
+ | [[Category: Po Hsun W]] |
Current revision
Structure of the (6-4) photolyase of Caulobacter crescentus in its oxidized state at room temperture-synchrotron
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