Structural highlights
Function
S26A9_MOUSE Ion transporter that can act both as an ion channel and anion exchanger (PubMed:15800055, PubMed:17120765, PubMed:31339488). Mainly acts as a chloride channel, which mediate uncoupled chloride anion transport in an alternate-access mechanism where a saturable binding site is alternately exposed to either one or the other side of the membrane (PubMed:31339488). Also acts as a DIDS- and thiosulfate- sensitive anion exchanger the exchange of chloride for bicarbonate ions across the cell membrane (PubMed:15800055).[1] [2] [3]
Publication Abstract from PubMed
The epithelial anion transporter SLC26A9 contributes to airway surface hydration and gastric acid production. Colocalizing with CFTR, SLC26A9 has been proposed as a target for the treatment of cystic fibrosis. To provide molecular details of its transport mechanism, we present cryo-EM structures and a functional characterization of murine Slc26a9. These structures define the general architecture of eukaryotic SLC26 family members and reveal an unusual mode of oligomerization which relies predominantly on the cytosolic STAS domain. Our data illustrates conformational transitions of Slc26a9, supporting a rapid alternate-access mechanism which mediates uncoupled chloride transport with negligible bicarbonate or sulfate permeability. The characterization of structure-guided mutants illuminates the properties of the ion transport path, including a selective anion binding site located in the center of a mobile module within the transmembrane domain. This study thus provides a structural foundation for the understanding of the entire SLC26 family and potentially facilitates their therapeutic exploitation.
Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport.,Walter JD, Sawicka M, Dutzler R Elife. 2019 Jul 24;8. pii: 46986. doi: 10.7554/eLife.46986. PMID:31339488[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Xu J, Henriksnas J, Barone S, Witte D, Shull GE, Forte JG, Holm L, Soleimani M. SLC26A9 is expressed in gastric surface epithelial cells, mediates Cl-/HCO3- exchange, and is inhibited by NH4+. Am J Physiol Cell Physiol. 2005 Aug;289(2):C493-505. Epub 2005 Mar 30. PMID:15800055 doi:http://dx.doi.org/00030.2005
- ↑ Romero MF, Chang MH, Plata C, Zandi-Nejad K, Mercado A, Broumand V, Sussman CR, Mount DB. Physiology of electrogenic SLC26 paralogues. Novartis Found Symp. 2006;273:126-38; discussion 138-47, 261-4 PMID:17120765
- ↑ Walter JD, Sawicka M, Dutzler R. Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport. Elife. 2019 Jul 24;8. pii: 46986. doi: 10.7554/eLife.46986. PMID:31339488 doi:http://dx.doi.org/10.7554/eLife.46986
- ↑ Walter JD, Sawicka M, Dutzler R. Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport. Elife. 2019 Jul 24;8. pii: 46986. doi: 10.7554/eLife.46986. PMID:31339488 doi:http://dx.doi.org/10.7554/eLife.46986
