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1wbp

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[[Image:1wbp.gif|left|200px]]
[[Image:1wbp.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1wbp |SIZE=350|CAPTION= <scene name='initialview01'>1wbp</scene>, resolution 2.40&Aring;
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The line below this paragraph, containing "STRUCTURE_1wbp", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Adp+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span>
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1wbp| PDB=1wbp | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wbp OCA], [http://www.ebi.ac.uk/pdbsum/1wbp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wbp RCSB]</span>
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'''SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE'''
'''SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE'''
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[[Category: Nolen, B.]]
[[Category: Nolen, B.]]
[[Category: Velazquez-Dones, A.]]
[[Category: Velazquez-Dones, A.]]
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[[Category: alternative splicing]]
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[[Category: Alternative splicing]]
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[[Category: atp-binding]]
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[[Category: Atp-binding]]
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[[Category: chromosome partition]]
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[[Category: Chromosome partition]]
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[[Category: differentiation]]
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[[Category: Differentiation]]
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[[Category: kinase]]
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[[Category: Kinase]]
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[[Category: mrna processing]]
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[[Category: Mrna processing]]
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[[Category: mrna splicing]]
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[[Category: Mrna splicing]]
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[[Category: nuclear protein]]
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[[Category: Nuclear protein]]
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[[Category: nucleotide-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: phosphorylation]]
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[[Category: Phosphorylation]]
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[[Category: serine/threonine-protein kinase]]
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[[Category: Serine/threonine-protein kinase]]
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[[Category: srpk]]
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[[Category: Srpk]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:25:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:33:59 2008''
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Revision as of 10:25, 3 May 2008

Image:1wbp.gif

Template:STRUCTURE 1wbp

SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE


Overview

The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases. However, the mode of phosphorylation by these kinases and their coordination in the biological regulation of ASF/SF2 is unknown. Here, we report the crystal structure of an active fragment of human SRPK1 bound to a peptide derived from an SR protein. This structure led us to identify a docking motif in ASF/SF2. We find that this docking motif restricts phosphorylation of ASF/SF2 by SRPK1 to the N-terminal part of the RS domain - a property essential for its assembly into nuclear speckles. We further show that Clk/Sty causes release of ASF/SF2 from speckles by phosphorylating the C-terminal part of its RS domain. These results suggest that the docking motif of ASF/SF2 is a key regulatory element for sequential phosphorylation by SRPK1 and Clk/Sty and, thus, is essential for its subcellular localization.

About this Structure

1WBP is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2., Ngo JC, Chakrabarti S, Ding JH, Velazquez-Dones A, Nolen B, Aubol BE, Adams JA, Fu XD, Ghosh G, Mol Cell. 2005 Oct 7;20(1):77-89. PMID:16209947 Page seeded by OCA on Sat May 3 13:25:52 2008

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