Horseradish peroxidase

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{{STRUCTURE_6atj| PDB=6atj | SIZE=400| SCENE= |right| CAPTION=Horseradish peroxidase containing heme group complex with ferulic acid and Ca+2 ion (green), [[6atj]] }}
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<StructureSection load='' size='350' side='right' caption='Horseradish peroxidase containing heme group complex with benzhydroxamic acid and Ca+2 ion (green) (PDB entry [[2atj]])' scene='48/486455/Cv/1'>
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== Function ==
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'''Horseradish peroxidase''' (HRP) catalyzes the oxidation of luminol to 3-aminophthalate using hydrogen peroxide. The reaction is accompanied by emission of light. In the presence of chemicals, particularly – p-iodophenol, the light emission is enhanced 1000-fold. Upon addition of the proper substrate, the HRP complex emits colored light. HRP contains a heme group<ref>PMID:14751298</ref>.
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== Relevance ==
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HRP isozyme C1a is widely used for detection of a labeled molecule to which it conjugates.
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'''Horseradish peroxidase''' (HRP) catalyzes the oxidation of luminol to 3-aminophthalate using hydrogen peroxide. The reaction is accompanied by emission of light. In the presence of chemicals, particularly – p-iodophenol, the light emission is enhanced 1000-fold. HRP isozyme C1a is widely used for detection of a labeled molecule to which it conjugates. Upon addition of the proper substrate, the HRP complex emits colored light. HRP contains a heme group.
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== Structural highlights ==
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The <scene name='48/486455/Cv/6'>active site contains a heme group and the substrate benzhydroxamic acid</scene><ref>PMID:9609699</ref>. <scene name='48/486455/Cv/7'>Fe coordination site</scene>. Water molecules shown as red spheres.
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</StructureSection>
==3D structures of horseradish peroxidase==
==3D structures of horseradish peroxidase==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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{{#tree:id=OrganizedByTopic|openlevels=0|
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[[1atj]], [[1h5d]], [[1h5e]], [[1h5f]], [[1h5g]], [[1g5i]], [[1g5j]], [[1g5k]], [[1g5m]], [[1g57]], [[1h55]], [[1h58]], [[1h5c]], [[1h5h]], [[1h5l]], [[1hch]] – HRP C1a<br />
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*Horseradish peroxidase
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[[1kzm]], [[1gwo]], [[1gwt]], [[1gwu]] - HRP C1a (mutant)
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'''Horseradish peroxidase binary complex'''
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**[[1atj]], [[1g57]], [[1g5i]], [[1g5j]], [[1g5k]], [[1g5m]], [[1h55]], [[1h57]], [[1h58]], [[1h5c]], [[1h5d]], [[1h5e]], [[1h5f]], [[1h5g]], [[1h5h]], [[1h5i]], [[1h5j]], [[1h5k]], [[1h5l]], [[1hch]], [[9h1m]] – HRP C1a<br />
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**[[1kzm]], [[1gwo]], [[1gwt]], [[1gwu]], [[2ylj]] - HRP C1a (mutant)
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[[2atj]] – HRP C1a + benzhydroxamic acid<br />
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*Horseradish peroxidase binary complex
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[[3atj]], [[4atj]], [[1gx2]] - HRP C1a (mutant) + benzhydroxamic acid<br />
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[[6atj]] - HRP C1a + ferulic acid<br />
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[[1gw2]] - HRP C1a (mutant) + ferulic acid<br />
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[[7atj]] - HRP C1a + CN + ferulic acid<br />
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[[1h5a]] – HRP C1a + acetate<br />
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[[1w4w]] - HRP C1a + formate<br />
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[[1w4w]] - HRP C1a + CO
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**[[2atj]] – HRP C1a + benzhydroxamic acid<br />
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**[[3atj]], [[4atj]], [[1gx2]] - HRP C1a (mutant) + benzhydroxamic acid<br />
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**[[6atj]] - HRP C1a + ferulic acid<br />
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**[[1gw2]] - HRP C1a (mutant) + ferulic acid<br />
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**[[7atj]] - HRP C1a + CN + ferulic acid<br />
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**[[1h5a]] – HRP C1a + acetate<br />
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**[[1w4w]] - HRP C1a + formate<br />
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**[[1w4y]] - HRP C1a + CO
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}}
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Horseradish peroxidase containing heme group complex with benzhydroxamic acid and Ca+2 ion (green) (PDB entry 2atj)

Drag the structure with the mouse to rotate

3D structures of horseradish peroxidase

Updated on 14-September-2025

References

  1. ↑ Veitch NC. Horseradish peroxidase: a modern view of a classic enzyme. Phytochemistry. 2004 Feb;65(3):249-59. PMID:14751298
  2. ↑ Henriksen A, Schuller DJ, Meno K, Welinder KG, Smith AT, Gajhede M. Structural interactions between horseradish peroxidase C and the substrate benzhydroxamic acid determined by X-ray crystallography. Biochemistry. 1998 Jun 2;37(22):8054-60. PMID:9609699 doi:10.1021/bi980234j

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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