Horseradish peroxidase
From Proteopedia
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| - | + | <StructureSection load='' size='350' side='right' caption='Horseradish peroxidase containing heme group complex with benzhydroxamic acid and Ca+2 ion (green) (PDB entry [[2atj]])' scene='48/486455/Cv/1'> | |
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== Function == | == Function == | ||
'''Horseradish peroxidase''' (HRP) catalyzes the oxidation of luminol to 3-aminophthalate using hydrogen peroxide. The reaction is accompanied by emission of light. In the presence of chemicals, particularly – p-iodophenol, the light emission is enhanced 1000-fold. Upon addition of the proper substrate, the HRP complex emits colored light. HRP contains a heme group<ref>PMID:14751298</ref>. | '''Horseradish peroxidase''' (HRP) catalyzes the oxidation of luminol to 3-aminophthalate using hydrogen peroxide. The reaction is accompanied by emission of light. In the presence of chemicals, particularly – p-iodophenol, the light emission is enhanced 1000-fold. Upon addition of the proper substrate, the HRP complex emits colored light. HRP contains a heme group<ref>PMID:14751298</ref>. | ||
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== Relevance == | == Relevance == | ||
HRP isozyme C1a is widely used for detection of a labeled molecule to which it conjugates. | HRP isozyme C1a is widely used for detection of a labeled molecule to which it conjugates. | ||
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| + | == Structural highlights == | ||
| + | The <scene name='48/486455/Cv/6'>active site contains a heme group and the substrate benzhydroxamic acid</scene><ref>PMID:9609699</ref>. <scene name='48/486455/Cv/7'>Fe coordination site</scene>. Water molecules shown as red spheres. | ||
</StructureSection> | </StructureSection> | ||
==3D structures of horseradish peroxidase== | ==3D structures of horseradish peroxidase== | ||
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*Horseradish peroxidase | *Horseradish peroxidase | ||
| - | **[[1atj]], [[ | + | **[[1atj]], [[1g57]], [[1g5i]], [[1g5j]], [[1g5k]], [[1g5m]], [[1h55]], [[1h57]], [[1h58]], [[1h5c]], [[1h5d]], [[1h5e]], [[1h5f]], [[1h5g]], [[1h5h]], [[1h5i]], [[1h5j]], [[1h5k]], [[1h5l]], [[1hch]], [[9h1m]] – HRP C1a<br /> |
**[[1kzm]], [[1gwo]], [[1gwt]], [[1gwu]], [[2ylj]] - HRP C1a (mutant) | **[[1kzm]], [[1gwo]], [[1gwt]], [[1gwu]], [[2ylj]] - HRP C1a (mutant) | ||
Current revision
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3D structures of horseradish peroxidase
Updated on 14-September-2025
References
- ↑ Veitch NC. Horseradish peroxidase: a modern view of a classic enzyme. Phytochemistry. 2004 Feb;65(3):249-59. PMID:14751298
- ↑ Henriksen A, Schuller DJ, Meno K, Welinder KG, Smith AT, Gajhede M. Structural interactions between horseradish peroxidase C and the substrate benzhydroxamic acid determined by X-ray crystallography. Biochemistry. 1998 Jun 2;37(22):8054-60. PMID:9609699 doi:10.1021/bi980234j
