1wu4

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[[Image:1wu4.gif|left|200px]]
[[Image:1wu4.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1wu4 |SIZE=350|CAPTION= <scene name='initialview01'>1wu4</scene>, resolution 1.35&Aring;
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The line below this paragraph, containing "STRUCTURE_1wu4", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Oligosaccharide_reducing-end_xylanase Oligosaccharide reducing-end xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.156 3.2.1.156] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= BH2105 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria])
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-->
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|DOMAIN=
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{{STRUCTURE_1wu4| PDB=1wu4 | SCENE= }}
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|RELATEDENTRY=[[1wu5|1WU5]], [[1wu6|1WU6]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wu4 OCA], [http://www.ebi.ac.uk/pdbsum/1wu4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wu4 RCSB]</span>
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}}
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'''Crystal structure of reducing-end-xylose releasing exo-oligoxylanase'''
'''Crystal structure of reducing-end-xylose releasing exo-oligoxylanase'''
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[[Category: Shoun, H.]]
[[Category: Shoun, H.]]
[[Category: Wakagi, T.]]
[[Category: Wakagi, T.]]
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[[Category: (alpla/alpha)6 barrel]]
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[[Category: Glycoside hydrolase family 8]]
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[[Category: glycoside hydrolase family 8]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:08:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:41:04 2008''
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Revision as of 11:08, 3 May 2008

Image:1wu4.gif

Template:STRUCTURE 1wu4

Crystal structure of reducing-end-xylose releasing exo-oligoxylanase


Overview

Reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125 (Rex) hydrolyzes xylooligosaccharides whose degree of polymerization is greater than or equal to 3, releasing the xylose unit at the reducing end. It is a unique exo-type glycoside hydrolase that recognizes the xylose unit at the reducing end in a very strict manner, even discriminating the beta-anomeric hydroxyl configuration from the alpha-anomer or 1-deoxyxylose. We have determined the crystal structures of Rex in unliganded and complex forms at 1.35-2.20-A resolution and revealed the structural aspects of its three subsites ranging from -2 to +1. The structure of Rex was compared with those of endo-type enzymes in glycoside hydrolase subfamily 8a (GH-8a). The catalytic machinery of Rex is basically conserved with other GH-8a enzymes. However, subsite +2 is blocked by a barrier formed by a kink in the loop before helix alpha10. His-319 in this loop forms a direct hydrogen bond with the beta-hydroxyl of xylose at subsite +1, contributing to the specific recognition of anomers at the reducing end.

About this Structure

1WU4 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125., Fushinobu S, Hidaka M, Honda Y, Wakagi T, Shoun H, Kitaoka M, J Biol Chem. 2005 Apr 29;280(17):17180-6. Epub 2005 Feb 17. PMID:15718242 Page seeded by OCA on Sat May 3 14:08:18 2008

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