9nq7

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Current revision (07:37, 12 November 2025) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 9nq7 is ON HOLD until Paper Publication
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==Cryo-EM structure of Csm/AcrIIIA2/enolase 4:3 complex==
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<StructureSection load='9nq7' size='340' side='right'caption='[[9nq7]], [[Resolution|resolution]] 2.72&Aring;' scene=''>
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Authors:
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== Structural highlights ==
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<table><tr><td colspan='2'>[[9nq7]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_phage_vB_SthS-VA214 Streptococcus phage vB_SthS-VA214] and [https://en.wikipedia.org/wiki/Streptococcus_thermophilus Streptococcus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NQ7 FirstGlance]. <br>
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Description:
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.72&#8491;</td></tr>
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[[Category: Unreleased Structures]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9nq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9nq7 OCA], [https://pdbe.org/9nq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9nq7 RCSB], [https://www.ebi.ac.uk/pdbsum/9nq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9nq7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CSM5_STRTR CSM5_STRTR] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities. In a heterologous host this Csm effector complex restricts ssRNA phage MS2, suggesting it may target RNA viruses in vivo.<ref>PMID:25458845</ref> Csm functions as a non-specific ssDNase. Base-pairing between crRNA and target RNA to form a ternary Csm complex activates a ssDNase activity; target RNA cleavage suppresses the ssDNase, a temporal control that prevents uncontrolled DNA degradation. Viral RNA transcripts probably tether the Csm complex to the viral genome, recruiting Cas10 ssDNA activity which is able to degrade DNA in the transcription bubble, spatially controlling the DNase activity.<ref>PMID:27105119</ref> This subunit might be involved in maturation of a crRNA intermediate to its mature form.<ref>PMID:25458845</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptococcus phage vB_SthS-VA214]]
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[[Category: Streptococcus thermophilus]]
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[[Category: Goswami HN]]
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[[Category: Li H]]

Current revision

Cryo-EM structure of Csm/AcrIIIA2/enolase 4:3 complex

PDB ID 9nq7

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