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| | ==Crystal structure of Coclaurine N-Methyltransferase (CNMT) bound to N-methylheliamine and SAH== | | ==Crystal structure of Coclaurine N-Methyltransferase (CNMT) bound to N-methylheliamine and SAH== |
| - | <StructureSection load='6gky' size='340' side='right' caption='[[6gky]], [[Resolution|resolution]] 2.85Å' scene=''> | + | <StructureSection load='6gky' size='340' side='right'caption='[[6gky]], [[Resolution|resolution]] 2.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6gky]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Copja Copja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GKY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6gky]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coptis_japonica Coptis japonica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GKY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F2Z:6,7-dimethoxy-2,4-dihydro-1~{H}-isoquinolin-3-one'>F2Z</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.847Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6gkv|6gkv]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F2Z:6,7-dimethoxy-2,4-dihydro-1~{H}-isoquinolin-3-one'>F2Z</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cnmt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3442 COPJA])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gky OCA], [https://pdbe.org/6gky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gky RCSB], [https://www.ebi.ac.uk/pdbsum/6gky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gky ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/(RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline_N-methyltransferase (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.115 2.1.1.115] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gky OCA], [http://pdbe.org/6gky PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gky RCSB], [http://www.ebi.ac.uk/pdbsum/6gky PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gky ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q948P7_COPJA Q948P7_COPJA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Copja]] | + | [[Category: Coptis japonica]] |
| - | [[Category: Dunstan, M S]] | + | [[Category: Large Structures]] |
| - | [[Category: Levy, C W]] | + | [[Category: Dunstan MS]] |
| - | [[Category: Coclaurine n-methyltransferase]] | + | [[Category: Levy CW]] |
| - | [[Category: N-methylheliamine]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
Q948P7_COPJA
Publication Abstract from PubMed
Benzylisoquinoline alkaloids (BIAs) are a structurally diverse family of plant secondary metabolites which have been exploited to develop analgesics, antibiotics, antitumor agents and other therapeutic agents. Biosynthesis of BIAs proceeds via a common pathway from tyrosine to (S)-reticulene at which point the pathway diverges. Coclaurine N-methyltransferase (CNMT) is a key enzyme in the pathway to (S)-reticulene, installing the N-methyl substituent that is essential for the bioactivity of many BIAs. In this paper, we describe the first crystal structure of CNMT which, along with mutagenesis studies, defines the enzymes active site architecture. The specificity of CNMT was also explored with a range of natural and synthetic substrates as well as co-factor analogues. Knowledge from this study could be used to generate improved CNMT variants required to produce BIAs or synthetic derivatives.
Structure and Biocatalytic Scope of Coclaurine N-Methyltransferase.,Bennett M, Thompson M, Shepherd S, Dunstan M, Herbert A, Smith D, Cronin V, Menon B, Levy C, Micklefield J Angew Chem Int Ed Engl. 2018 May 23. doi: 10.1002/anie.201805060. PMID:29791083[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bennett M, Thompson M, Shepherd S, Dunstan M, Herbert A, Smith D, Cronin V, Menon B, Levy C, Micklefield J. Structure and Biocatalytic Scope of Coclaurine N-Methyltransferase. Angew Chem Int Ed Engl. 2018 May 23. doi: 10.1002/anie.201805060. PMID:29791083 doi:http://dx.doi.org/10.1002/anie.201805060
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