1xkz
From Proteopedia
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'''Crystal structure of the acylated beta-lactam sensor domain of Blar1 from S. aureus''' | '''Crystal structure of the acylated beta-lactam sensor domain of Blar1 from S. aureus''' | ||
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[[Category: Schlegel, H B.]] | [[Category: Schlegel, H B.]] | ||
[[Category: Schulze-Briese, C.]] | [[Category: Schulze-Briese, C.]] | ||
| - | [[Category: | + | [[Category: Beta-lactam receptor]] |
| - | [[Category: | + | [[Category: Signal transduction]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:10:00 2008'' | |
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Revision as of 12:10, 3 May 2008
Crystal structure of the acylated beta-lactam sensor domain of Blar1 from S. aureus
Overview
Methicillin-resistant strains of Staphylococcus aureus (MRSA) are the major cause of infections worldwide. Transcription of the beta-lactamase and PBP2a resistance genes is mediated by two closely related signal-transducing integral membrane proteins, BlaR1 and MecR1, upon binding of the beta-lactam inducer to the sensor domain. Herein we report the crystal structure at 1.75 A resolution of the sensor domain of BlaR1 in complex with a cephalosporin antibiotic. Activation of the signal transducer involves acylation of serine 389 by the beta-lactam antibiotic, a process promoted by the N-carboxylated side chain of Lys392. We present evidence that, on acylation, the lysine side chain experiences a spontaneous decarboxylation that entraps the sensor in its activated state. Kinetic determinations and quantum mechanical/molecular mechanical calculations and the interaction networks in the crystal structure shed light on how this unprecedented process for activation of a receptor may be achieved and provide insights into the mechanistic features that differentiate the signal-transducing receptor from the structurally related class D beta-lactamases, enzymes of antibiotic resistance.
About this Structure
1XKZ is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
X-ray crystal structure of the acylated beta-lactam sensor domain of BlaR1 from Staphylococcus aureus and the mechanism of receptor activation for signal transduction., Birck C, Cha JY, Cross J, Schulze-Briese C, Meroueh SO, Schlegel HB, Mobashery S, Samama JP, J Am Chem Soc. 2004 Nov 3;126(43):13945-7. PMID:15506754 Page seeded by OCA on Sat May 3 15:10:00 2008
