1yfh
From Proteopedia
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[[Image:1yfh.gif|left|200px]] | [[Image:1yfh.gif|left|200px]] | ||
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'''wt Human O6-Alkylguanine-DNA Alkyltransferase Bound To DNA Containing an Alkylated Cytosine''' | '''wt Human O6-Alkylguanine-DNA Alkyltransferase Bound To DNA Containing an Alkylated Cytosine''' | ||
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The structure of the human AGT protein bound to DNA and its implications for damage detection., Duguid EM, Rice PA, He C, J Mol Biol. 2005 Jul 22;350(4):657-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15964013 15964013] | The structure of the human AGT protein bound to DNA and its implications for damage detection., Duguid EM, Rice PA, He C, J Mol Biol. 2005 Jul 22;350(4):657-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15964013 15964013] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Methylated-DNA--[protein]-cysteine S-methyltransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Duguid, E M.]] | [[Category: Duguid, E M.]] | ||
[[Category: He, C.]] | [[Category: He, C.]] | ||
[[Category: Rice, P A.]] | [[Category: Rice, P A.]] | ||
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:15:24 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:15, 3 May 2008
wt Human O6-Alkylguanine-DNA Alkyltransferase Bound To DNA Containing an Alkylated Cytosine
Overview
O6-Alklyguanine-DNA alkyltransferase (AGT) is an important DNA repair protein that protects cells from mutagenesis and toxicity arising from alkylating agents. We present an X-ray crystal structure of the wild-type human protein (hAGT) bound to double-stranded DNA with a chemically modified cytosine base. The protein binds at two different sites: one at the modified base, and the other across a sticky-ended DNA junction. The protein molecule that binds the modified cytosine base flips the base and recognizes it in its active site. The one that binds ends of neighboring DNA molecules partially flips an overhanging thymine base. This base is not inserted into the active-site pocket of the protein. These two different hAGT/DNA interactions observed in the structure suggest that hAGT may not detect DNA lesions by searching for the adduct itself, but rather for weakened and/or distorted base-pairs caused by base damage in the duplex DNA. We propose that hAGT imposes a strain on the DNA duplex and searches for DNA regions where the native structure is destabilized. The structure provides implications for pyrimidine recognition, improved inhibitor design, and a possible protein/protein interaction patch on hAGT.
About this Structure
1YFH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The structure of the human AGT protein bound to DNA and its implications for damage detection., Duguid EM, Rice PA, He C, J Mol Biol. 2005 Jul 22;350(4):657-66. PMID:15964013 Page seeded by OCA on Sat May 3 16:15:24 2008
