2bz2
From Proteopedia
(New page: 200px<br /> <applet load="2bz2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bz2" /> '''SOLUTION STRUCTURE OF NELF E RRM'''<br /> ...) |
|||
| Line 6: | Line 6: | ||
==Overview== | ==Overview== | ||
The elongation of transcription of HIV RNA at the TAR, (transactivation-response element) is highly regulated by positive and, negative factors. The cellular negative transcription elongation factor, NELF (negative elongation factor) was suggested to be involved in, transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of, the viral TAR RNA which is synthesized by cellular RNA polymerase II at, the viral long terminal repeat. NELF is a heterotetrameric protein, consisting of NELF A, B, C or the splice variant D, and E. In the present, study, we determined the solution structure of the RRM (RNA-recognition, motif) of the RNA-binding subunit NELF E and studied its interaction with, the viral TAR RNA. Our results show that the separately expressed, recombinant NELF E RRM has alpha-helical and beta-strand elements adopting, a betaalphabetabetaalphabeta fold and is able to bind to TAR RNA., Fluorescence equilibrium titrations with fluorescently labelled double-, and single-stranded oligoribonucleotides representing the TAR RNA stem, imply that NELF E RRM binds to the single-stranded TAR RNAs with K(d), values in the low-micromolar range. | The elongation of transcription of HIV RNA at the TAR, (transactivation-response element) is highly regulated by positive and, negative factors. The cellular negative transcription elongation factor, NELF (negative elongation factor) was suggested to be involved in, transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of, the viral TAR RNA which is synthesized by cellular RNA polymerase II at, the viral long terminal repeat. NELF is a heterotetrameric protein, consisting of NELF A, B, C or the splice variant D, and E. In the present, study, we determined the solution structure of the RRM (RNA-recognition, motif) of the RNA-binding subunit NELF E and studied its interaction with, the viral TAR RNA. Our results show that the separately expressed, recombinant NELF E RRM has alpha-helical and beta-strand elements adopting, a betaalphabetabetaalphabeta fold and is able to bind to TAR RNA., Fluorescence equilibrium titrations with fluorescently labelled double-, and single-stranded oligoribonucleotides representing the TAR RNA stem, imply that NELF E RRM binds to the single-stranded TAR RNAs with K(d), values in the low-micromolar range. | ||
| + | |||
| + | ==Disease== | ||
| + | Known diseases associated with this structure: Blood group, Radin OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=609017 609017]], Blood group, Scianna system OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=609017 609017]] | ||
==About this Structure== | ==About this Structure== | ||
| Line 30: | Line 33: | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 21:08:15 2007'' |
Revision as of 19:01, 12 November 2007
|
SOLUTION STRUCTURE OF NELF E RRM
Contents |
Overview
The elongation of transcription of HIV RNA at the TAR, (transactivation-response element) is highly regulated by positive and, negative factors. The cellular negative transcription elongation factor, NELF (negative elongation factor) was suggested to be involved in, transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of, the viral TAR RNA which is synthesized by cellular RNA polymerase II at, the viral long terminal repeat. NELF is a heterotetrameric protein, consisting of NELF A, B, C or the splice variant D, and E. In the present, study, we determined the solution structure of the RRM (RNA-recognition, motif) of the RNA-binding subunit NELF E and studied its interaction with, the viral TAR RNA. Our results show that the separately expressed, recombinant NELF E RRM has alpha-helical and beta-strand elements adopting, a betaalphabetabetaalphabeta fold and is able to bind to TAR RNA., Fluorescence equilibrium titrations with fluorescently labelled double-, and single-stranded oligoribonucleotides representing the TAR RNA stem, imply that NELF E RRM binds to the single-stranded TAR RNAs with K(d), values in the low-micromolar range.
Disease
Known diseases associated with this structure: Blood group, Radin OMIM:[609017], Blood group, Scianna system OMIM:[609017]
About this Structure
2BZ2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription., Rao JN, Neumann L, Wenzel S, Schweimer K, Rosch P, Wohrl BM, Biochem J. 2006 Dec 15;400(3):449-56. PMID:16898873
Page seeded by OCA on Mon Nov 12 21:08:15 2007
