1z5s

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[[Image:1z5s.gif|left|200px]]
[[Image:1z5s.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1z5s |SIZE=350|CAPTION= <scene name='initialview01'>1z5s</scene>, resolution 3.01&Aring;
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The line below this paragraph, containing "STRUCTURE_1z5s", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= UBE2I, UBC9, UBCE9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), UBL1, SMT3C, SMT3H3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RANGAP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RANBP2, NUP358 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1z5s| PDB=1z5s | SCENE= }}
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|RELATEDENTRY=[[1kps|1KPS]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z5s OCA], [http://www.ebi.ac.uk/pdbsum/1z5s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z5s RCSB]</span>
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}}
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'''Crystal structure of a complex between UBC9, SUMO-1, RANGAP1 and NUP358/RANBP2'''
'''Crystal structure of a complex between UBC9, SUMO-1, RANGAP1 and NUP358/RANBP2'''
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[[Category: Lima, C D.]]
[[Category: Lima, C D.]]
[[Category: Reverter, D.]]
[[Category: Reverter, D.]]
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[[Category: e3]]
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[[Category: E3]]
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[[Category: ligase]]
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[[Category: Ligase]]
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[[Category: nuclear pore complex]]
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[[Category: Nuclear pore complex]]
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[[Category: sumo]]
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[[Category: Sumo]]
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[[Category: ubc9]]
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[[Category: Ubc9]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:12:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:29:26 2008''
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Revision as of 14:12, 3 May 2008

Image:1z5s.gif

Template:STRUCTURE 1z5s

Crystal structure of a complex between UBC9, SUMO-1, RANGAP1 and NUP358/RANBP2


Overview

SUMO-1 (for small ubiquitin-related modifier) belongs to the ubiquitin (Ub) and ubiquitin-like (Ubl) protein family. SUMO conjugation occurs on specific lysine residues within protein targets, regulating pathways involved in differentiation, apoptosis, the cell cycle and responses to stress by altering protein function through changes in activity or cellular localization or by protecting substrates from ubiquitination. Ub/Ubl conjugation occurs in sequential steps and requires the concerted action of E2 conjugating proteins and E3 ligases. In addition to being a SUMO E3, the nucleoporin Nup358/RanBP2 localizes SUMO-conjugated RanGAP1 to the cytoplasmic face of the nuclear pore complex by means of interactions in a complex that also includes Ubc9, the SUMO E2 conjugating protein. Here we describe the 3.0-A crystal structure of a four-protein complex of Ubc9, a Nup358/RanBP2 E3 ligase domain (IR1-M) and SUMO-1 conjugated to the carboxy-terminal domain of RanGAP1. Structural insights, combined with biochemical and kinetic data obtained with additional substrates, support a model in which Nup358/RanBP2 acts as an E3 by binding both SUMO and Ubc9 to position the SUMO-E2-thioester in an optimal orientation to enhance conjugation.

About this Structure

1Z5S is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex., Reverter D, Lima CD, Nature. 2005 Jun 2;435(7042):687-92. PMID:15931224 Page seeded by OCA on Sat May 3 17:12:20 2008

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