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229d

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[[Image:229d.gif|left|200px]]
[[Image:229d.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 229d |SIZE=350|CAPTION= <scene name='initialview01'>229d</scene>
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The line below this paragraph, containing "STRUCTURE_229d", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=5CM:5-METHYL-2&#39;-DEOXY-CYTIDINE-5&#39;-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=MG1:2&#39;-DEOXY-1-METHYLGUANOSINE+5&#39;-(DIHYDROGEN+PHOSPHATE)'>MG1</scene>, <scene name='pdbligand=UMP:2&#39;-DEOXYURIDINE+5&#39;-MONOPHOSPHATE'>UMP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_229d| PDB=229d | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=229d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=229d OCA], [http://www.ebi.ac.uk/pdbsum/229d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=229d RCSB]</span>
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}}
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'''DNA ANALOG OF YEAST TRANSFER RNA PHE ANTICODON DOMAIN WITH MODIFIED BASES 5-METHYL CYTOSINE AND 1-METHYL GUANINE'''
'''DNA ANALOG OF YEAST TRANSFER RNA PHE ANTICODON DOMAIN WITH MODIFIED BASES 5-METHYL CYTOSINE AND 1-METHYL GUANINE'''
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==About this Structure==
==About this Structure==
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229D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=229D OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=229D OCA].
==Reference==
==Reference==
Design, biological activity and NMR-solution structure of a DNA analogue of yeast tRNA(Phe) anticodon domain., Basti MM, Stuart JW, Lam AT, Guenther R, Agris PF, Nat Struct Biol. 1996 Jan;3(1):38-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8548453 8548453]
Design, biological activity and NMR-solution structure of a DNA analogue of yeast tRNA(Phe) anticodon domain., Basti MM, Stuart JW, Lam AT, Guenther R, Agris PF, Nat Struct Biol. 1996 Jan;3(1):38-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8548453 8548453]
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[[Category: Protein complex]]
 
[[Category: Agris, P F.]]
[[Category: Agris, P F.]]
[[Category: Basti, M M.]]
[[Category: Basti, M M.]]
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[[Category: Lam, A T.]]
[[Category: Lam, A T.]]
[[Category: Stuart, J W.]]
[[Category: Stuart, J W.]]
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[[Category: anticodon]]
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[[Category: Anticodon]]
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[[Category: dna]]
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[[Category: Dna]]
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[[Category: hairpin loop]]
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[[Category: Hairpin loop]]
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[[Category: transfer rna]]
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[[Category: Transfer rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:20:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:44:43 2008''
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Revision as of 15:20, 3 May 2008

Image:229d.gif

Template:STRUCTURE 229d

DNA ANALOG OF YEAST TRANSFER RNA PHE ANTICODON DOMAIN WITH MODIFIED BASES 5-METHYL CYTOSINE AND 1-METHYL GUANINE


Overview

Design of biologically active DNA analogues of the yeast tRNA(Phe) anticodon domain, tDNAPheAC, required the introduction of a d(m5C)-dependent, Mg(2+)-induced structural transition and the d(m1G) disruption of an intra-loop dC.dG base pair. The modifications were introduced at residues corresponding to m5C-40 and wybutosine-37 in tRNA(Phe). Modified tDNAPheAC inhibited translation by 50% at a tDNAPheAC:ribosome ratio of 8:1. The molecule's structure has been determined by NMR spectroscopy and restrained molecular dynamics with an overall r.m.s.d. of 2.8 A and 1.7 A in the stem, and is similar to the tRNA(Phe) anticodon domain in conformation and dimensions. The tDNAPheAC structure may provide a guide for the design of translation inhibitors as potential therapeutic agents.

About this Structure

Full crystallographic information is available from OCA.

Reference

Design, biological activity and NMR-solution structure of a DNA analogue of yeast tRNA(Phe) anticodon domain., Basti MM, Stuart JW, Lam AT, Guenther R, Agris PF, Nat Struct Biol. 1996 Jan;3(1):38-44. PMID:8548453 Page seeded by OCA on Sat May 3 18:20:38 2008

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