2a5a
From Proteopedia
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'''Crystal structure of unbound SARS coronavirus main peptidase in the space group C2''' | '''Crystal structure of unbound SARS coronavirus main peptidase in the space group C2''' | ||
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[[Category: Powers, J C.]] | [[Category: Powers, J C.]] | ||
[[Category: 3c-like]] | [[Category: 3c-like]] | ||
| - | [[Category: | + | [[Category: Alpha-helical domain]] |
| - | [[Category: | + | [[Category: Catalytic dyad]] |
| - | [[Category: | + | [[Category: Chymotrypsin-like fold]] |
| - | [[Category: | + | [[Category: Cysteine peptidase]] |
| - | [[Category: | + | [[Category: Dimer]] |
| - | [[Category: | + | [[Category: Long loop]] |
| - | [[Category: | + | [[Category: N-finger]] |
| - | [[Category: | + | [[Category: Specificity pocket]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:37:23 2008'' | |
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Revision as of 15:37, 3 May 2008
Crystal structure of unbound SARS coronavirus main peptidase in the space group C2
Overview
The main peptidase (M(pro)) from the coronavirus (CoV) causing severe acute respiratory syndrome (SARS) is one of the most attractive molecular targets for the development of anti-SARS agents. We report the irreversible inhibition of SARS-CoV M(pro) by an aza-peptide epoxide (APE; k(inact)/K(i) = 1900(+/-400) M(-1) s(-1)). The crystal structures of the M(pro):APE complex in the space groups C2 and P2(1)2(1)2(1) revealed the formation of a covalent bond between the catalytic Cys145 S(gamma) atom of the peptidase and the epoxide C3 atom of the inhibitor, substantiating the mode of action of this class of cysteine-peptidase inhibitors. The aza-peptide component of APE binds in the substrate-binding regions of M(pro) in a substrate-like manner, with excellent structural and chemical complementarity. In addition, the crystal structure of unbound M(pro) in the space group C2 revealed that the "N-fingers" (N-terminal residues 1 to 7) of both protomers of M(pro) are well defined and the substrate-binding regions of both protomers are in the catalytically competent conformation at the crystallization pH of 6.5, contrary to the previously determined crystal structures of unbound M(pro) in the space group P2(1).
About this Structure
2A5A is a Single protein structure of sequence from Human sars coronavirus. Full crystallographic information is available from OCA.
Reference
Crystal structures of the main peptidase from the SARS coronavirus inhibited by a substrate-like aza-peptide epoxide., Lee TW, Cherney MM, Huitema C, Liu J, James KE, Powers JC, Eltis LD, James MN, J Mol Biol. 2005 Nov 11;353(5):1137-51. Epub 2005 Sep 27. PMID:16219322 Page seeded by OCA on Sat May 3 18:37:23 2008
Categories: Human sars coronavirus | Single protein | Cherney, M M. | Eltis, L D. | Huitema, C. | James, K E. | James, M N. | Lee, T W. | Liu, J. | Powers, J C. | 3c-like | Alpha-helical domain | Catalytic dyad | Chymotrypsin-like fold | Cysteine peptidase | Dimer | Long loop | N-finger | Specificity pocket
