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2a7o

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[[Image:2a7o.gif|left|200px]]
[[Image:2a7o.gif|left|200px]]
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{{Structure
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|GENE= hSet2/HYPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7o OCA], [http://www.ebi.ac.uk/pdbsum/2a7o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a7o RCSB]</span>
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'''Solution Structure of the hSet2/HYPB SRI domain'''
'''Solution Structure of the hSet2/HYPB SRI domain'''
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[[Category: Sage, H.]]
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[[Category: phosphoctd associating protein]]
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[[Category: Phosphoctd associating protein]]
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[[Category: set2]]
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[[Category: Set2]]
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[[Category: set2 rpb1-interacting domain]]
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[[Category: Set2 rpb1-interacting domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:42:44 2008''
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Revision as of 15:42, 3 May 2008

Image:2a7o.gif

Template:STRUCTURE 2a7o

Solution Structure of the hSet2/HYPB SRI domain


Overview

The phosphorylation state of the C-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II changes as polymerase transcribes a gene, and the distinct forms of the phospho-CTD (PCTD) recruit different nuclear factors to elongating polymerase. The Set2 histone methyltransferase from yeast was recently shown to bind the PCTD of elongating RNA polymerase II by means of a novel domain termed the Set2-Rpb1 interacting (SRI) domain. Here, we report the solution structure of the SRI domain in human Set2 (hSRI domain), which adopts a left-turned three-helix bundle distinctly different from other structurally characterized PCTD-interacting domains. NMR titration experiments mapped the binding surface of the hSRI domain to helices 1 and 2, and Biacore binding studies showed that the domain binds preferably to [Ser-2 + Ser-5]-phosphorylated CTD peptides containing two or more heptad repeats. Point-mutagenesis studies identified five residues critical for PCTD binding. In view of the differential effects of these point mutations on binding to different CTD phosphopeptides, we propose a model for the hSRI domain interaction with the PCTD.

About this Structure

2A7O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1., Li M, Phatnani HP, Guan Z, Sage H, Greenleaf AL, Zhou P, Proc Natl Acad Sci U S A. 2005 Dec 6;102(49):17636-41. Epub 2005 Nov 28. PMID:16314571 Page seeded by OCA on Sat May 3 18:42:44 2008

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